Reversion by polyclonal antibodies of α effects of Tityus serrulatus venom on frog sciatic nerve

• Published in: Toxicology letters Vol. 76; no. 3; pp. 187 - 193
• Main Authors: Silveira, Josianne N., Heneine, Ibrahim F., Beirão, Paulo S.L.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01.04.1995; Amsterdam Elsevier Science
• Subjects: Action Potentials - drug effects; Animal poisons toxicology. Antivenoms; Animals; Biological and medical sciences; Buthidae; Electrophysiology; Enzyme-Linked Immunosorbent Assay; gamma-Globulins - immunology; Immunoglobulin G - immunology; In Vitro Techniques; Medical sciences; Neurotoxins - immunology; Neurotoxins - toxicity; Polyclonal antibody; Rabbits; Rana catesbeiana; Sciatic Nerve - drug effects; Scorpion venom; Scorpion Venoms - immunology; Scorpion Venoms - toxicity; Scorpiones; Sodium channel; Sodium Channels - drug effects; Tityus serrulatus; Toxicology; Toxin deactivation; Sodium channel; Scorpion venom; Polyclonal antibody; Toxin deactivation; Scorpionides; Nervous system diseases; Frog; Toxicity; Amphibia; Electrophysiology; Animal origin; Action potential; Salientia; Ionic channel; In vitro; Arachnida; Vertebrata; Treatment; Arthropoda; Animal; Immunotherapy; Venom; Invertebrata; Antidote; Sciatic nerve
• ISSN: 0378-4274; 1879-3169
• DOI: 10.1016/0378-4274(95)80002-U

Fraction T 2 from Tityus serrulatus venom produced a marked lengthening of action potentials recorded with the single sucrose-gap technique, a characteristic effect of α scorpion toxins. This effect was not reversed by thorough washing of the nerve. On the other hand, T 2 fraction deactivated by complete iodination did not cause any alteration of the compound action potential, even if applied in concentration as high as 4000 times the half saturation dose of the unmodified T 2 fraction. This high dose of deactivated T 2 did not hinder the onset of the full effect of a single dose of T 2 fraction applied subsequently. Polyclonal antibodies against native or against iodinated venom reverted the action of T 2 fraction, restoring normal electrical response. We conclude that both types of antibodies may remove the effect of α toxins on sodium channel.