Ovalbumin is more immunogenic when introduced into brain or cerebrospinal fluid than into extracerebral sites

• Published in: Journal of neuroimmunology Vol. 40; no. 1; pp. 81 - 87
• Main Authors: Gordon, Leslie B., Knopf, Paul M., Cserr, Helen F.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: London Elsevier B.V 01.09.1992; Amsterdam Elsevier; New York, NY
• Subjects: Administration, Intranasal; Analysis of the immune response. Humoral and cellular immunity; Animals; Antibodies - analysis; Antibody; Antibody production; Biological and medical sciences; Blood - immunology; Blood-brain barrier; Brain - immunology; Caudate Nucleus - immunology; Central nervous system immunization; Cerebral Ventricles - immunology; Cerebrospinal Fluid - immunology; Cervical lymph node; Drug Combinations; Foot; Freund's Adjuvant - immunology; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humoral immune response; Immunization - methods; Immunobiology; Injections; Injections, Intravenous; Male; Ovalbumin - administration & dosage; Ovalbumin - immunology; Rats; Rats, Inbred Strains; T-dependent antigens; Humoral immune response; T-dependent antigens; Cervical lymph node; Blood-brain barrier; Central nervous system immunization; Antibody; Ovalbumin; Immunization; Immune response; Rat; Rodentia; Glycoproteins; Cerebrospinal fluid; Route of administration; Vertebrata; Mammalia; Immunogenicity; Intracerebral administration; Comparative study; Humoral immunity
• ISSN: 0165-5728; 1872-8421
• DOI: 10.1016/0165-5728(92)90215-7

The magnitudes of serum antibody responses to ovalbumin have been compared following immunization via cerebral or extracerebral sites in Sprague-Dawley rats. In central nervous system (CNS)-immunized rats, conditions were designed to ensure normal brain barrier permeability. Extracerebral immunization was via the footpad or along pathways of antigen outflow from the CNS. The relative immunogenicity of different injection sites is: CSF > brain tissus > extracerebral sites. Enhancement of the antibody response to CNS-administered antigen appears to depend on events initiated within the CNS, since ovalbumin injected into blood (which reaches the spleen) or nasal submucosa (which drains to cervical nodes) fails to elicit a similar response.