Intertypic differentiation and detection of intratypic variants among canine and phocid morbillivirus isolates by kinetic neutralization using a novel immunoplaque assay

• Published in: Journal of virological methods Vol. 41; no. 1; pp. 77 - 92
• Main Authors: Harder, T.C., Klusmeyer, K., Frey, H.-R., Örvell, C., Liess, B.
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 1993; Amsterdam Elsevier; New York, NY
• Subjects: Animals; Antibodies, Monoclonal; Antigens, Viral; Biological and medical sciences; Canine distemper virus; Distemper Virus, Canine - immunology; Distemper Virus, Canine - isolation & purification; Dogs; Evaluation Studies as Topic; Fundamental and applied biological sciences. Psychology; Immunoplaque assay; Kinetic neutralization; Measles virus - immunology; Measles virus - isolation & purification; Microbiology; Monoclonal antibody; Neutralization Tests - methods; Paramyxoviridae - classification; Paramyxoviridae - immunology; Paramyxoviridae - isolation & purification; Phocid distemper virus; Seals, Earless - microbiology; Techniques used in virology; Viral Plaque Assay - methods; Virology; Virology - methods; Kinetic neutralization; Phocid distemper virus; Monoclonal antibody; Canine distemper virus; Immunoplaque assay; Typing; Antigenic determinant; Antibody; Identification; Method; Enzyme immunoassay; Canine distemper morbillivirus; Paramyxoviridae; Virus; Neutralization test; Morbillivirus; Kinetics; Detection
• ISSN: 0166-0934; 1879-0984
• DOI: 10.1016/0166-0934(93)90164-M

Intertypic antigenic differences and the intratypic variability of the closely related canine (CVD) and phocid distemper viruses (PDV) were examined using a molecular (monoclonal antibodies specific for the H- and F-glycoproteins) and a functional (kinetic neutralization, KN) approach. KN studies were carried out using a novel immunoplaque technique which combined conventional plaque assay and antigen-specific enzyme-immuno-staining techniques. Morbillivirus isolates of canine and phocid origin, clearly formed two separate groups. Minor antigenic differences were also evident within each cluster. A distemper isolate of mustelid origin was distinguishable from both CDV- and PDV-like prototype viruses by kinetic neutralization.