A device for transplantation of single cells

A device with a high degree of precision is described for the transplantation of single identified cells in specified regions of the host brain. It consists of two co-centric micropipettes where the inner pipette, functioning as a plunger, is used to drive the cells contained near the tip of the out...

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Bibliographic Details
Published inJournal of neuroscience methods Vol. 49; no. 1; pp. 1 - 3
Main Authors Sharma, Shail K., Singh, U.N.
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 01.08.1993
Elsevier Science
Subjects
Animals
Biological and medical sciences
Brain
Brain transplantation
Cell transplantation
Cell Transplantation - instrumentation
Equipment Design
Fundamental and applied biological sciences. Psychology
General aspects. Models. Methods
Microinjection
Microinjections - instrumentation
Neuronal differentiation
Regional specificity in cell differentiation
Vertebrates: nervous system and sense organs
Neuronal differentiation
Microinjection
Regional specificity in cell differentiation
Brain transplantation
Cell transplantation
Isolated cell
Instrumentation
Transplantation
Technique
Central nervous system
Brain (vertebrata)
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Summary:A device with a high degree of precision is described for the transplantation of single identified cells in specified regions of the host brain. It consists of two co-centric micropipettes where the inner pipette, functioning as a plunger, is used to drive the cells contained near the tip of the outer pipette into the host. The use of a pre-calibrated micrometer to control the movement of the inner pipette obviates the need of visual monitoring for successful transplantation.
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ISSN:0165-0270
1872-678X
DOI:10.1016/0165-0270(93)90102-W