High level expression, purification, and characterization of the Kunitz-type protease inhibitor domain of protease nexin-2/amyloid β-protein precursor

• Published in: Biochemical and biophysical research communications Vol. 186; no. 2; pp. 1138 - 1145
• Main Authors: Wagner, Steven L., Siegel, Robert S., Vedvick, Thomas S., Raschke, William C., Van Nostrand, William E.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 31.07.1992; Elsevier
• Subjects: Amino Acid Sequence; amyloid beta -protein; Amyloid beta-Protein Precursor - genetics; Amyloid beta-Protein Precursor - isolation & purification; Amyloid beta-Protein Precursor - pharmacology; Analytical, structural and metabolic biochemistry; Base Sequence; Biological and medical sciences; Carrier Proteins - genetics; Carrier Proteins - isolation & purification; Carrier Proteins - pharmacology; Cells, Cultured; Cloning, Molecular; domains; Endopeptidases - metabolism; Enzymes and enzyme inhibitors; expression; Fundamental and applied biological sciences. Psychology; Humans; Hydrolases; Kinetics; Molecular Sequence Data; nexin-2; Pichia - genetics; Pichia pastoris; Plasmids; precursors; Protease Nexins; proteinase inhibitor; purification; Receptors, Cell Surface; Recombinant Proteins - isolation & purification; Recombinant Proteins - pharmacology; Restriction Mapping; Trypsin Inhibitor, Kunitz Soybean - genetics; Trypsin Inhibitor, Kunitz Soybean - pharmacology; use; Aβ; AβPP; KPI; PN-2; αMF; Vertebrata; Yeast; Mammalia; Purification; Enzyme; Proteinase; Inhibition constant; Enzyme inhibitor; Recombinant protein; Gene expression; Amyloid precursor protein
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/0006-291X(92)90865-I

The protease inhibitor, protease nexin-2 (PN-2), is the secreted isoform of the Alzheimer's amyloid β-protein precursor (AβPP) that contains the Kunitz-type protease inhibitor (KPI) domain. Here we describe the use of the methylotrophic industrial yeast Pichia pastoris as a host system for the large scale production of the KPI domain of PN-2 AβPP . In addition to the 57 amino acid KPI domain, the expression product contained an additional four amino acid residues at its amino terminus that correspond to amino acids 285–288 of AβPP (Ponte et al. 1988 Nature 311:525–527). This expression system generated yields of greater than 1.0 gram of KPI domain per liter of fermentation media. The secreted 61 amino acid product was purified to homogeneity and biochemically characterized. Amino acid analysis and sequencing of the entire expressed KPI domain verified its integrity. Similar to native PN-2 AβPP , the purified KPI domain potently inhibited trypsin, chymotrypsin, and coagulation factor XIa. Although heparin augments the inhibition of factor XIa by native PN-2 AβPP it had no effect on the inhibition of factor XIa by expressed KPI domain suggesting that heparin binds to regions on native PN-2 AβPP outside of the protease inhibitory domain. This KPI domain expression product should be useful in studying the physiologic and pathophysiologic functions of PN-2 AβPP .