Human α 1-acid glycoprotein-exposed macrophages release interleukin 1 inhibitory activity

• Published in: Biochemical and biophysical research communications Vol. 147; no. 2; pp. 710 - 715
• Main Authors: Bories, P.N., Guenounou, M., Féger, J., Kodari, E., Agneray, J., Durand, G.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 15.09.1987; Elsevier
• Subjects: Animals; Biological and medical sciences; Cell Division; Cells, Cultured; Electrophoresis, Polyacrylamide Gel; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; Immunobiology; Interleukin-1 - physiology; Lymphokines - metabolism; Lymphokines - pharmacology; Macrophages - drug effects; Macrophages - metabolism; Mice; Mice, Inbred C3H; Mice, Inbred DBA; Modulation of the immune response (stimulation, suppression); Monocytes - drug effects; Monocytes - metabolism; Orosomucoid - metabolism; Orosomucoid - pharmacology; Peritoneal Cavity - cytology; Thymus Gland - cytology; Hanks'balanced salt solution; Con A; AGP; Concanavalin A; TNF; IL2; IL1; SDS-PAGE; tumor necrosis factor; Interleukin 2; Interleukin 1; FCS; sodium dodecylsulfate-polyacrylamide gel electrophoresis; fetal calf serum; α 1-acid glycoprotein; HBSS; Human; Culture medium; Cell proliferation; Cell culture; Monocyte; Immunoaffinity chromatography; Immunomodulation; Thymocyte; Monokine; Activation; Cellular immunity; Immune regulation; Biological activity; Affinity chromatography; Seromucoid; Inhibitor; Inhibition; Macrophage
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/0006-291X(87)90988-0

The concanavalin A-unreactive variants of α 1-acid glycoprotein introduced in culture medium of monocytes/macrophages induces the inhibition of thymocyte proliferative activity of interleukin 1. LPS or LPS receptors were not involved in the activity of α 1-acid glycoprotein on macrophages. α 1- acid glycoprotein did not show any direct effect on thymocytes whereas the monocyte/macrophage-supernatant inhibited the interleukin 1 proliferative effect. The activity of tumor necrosis factor and interleukin 2 was not altered by the α 1- acid glycoprotein-macrophage supernatant.