Identification and characterization of tox21A: a mite cDNA encoding a paralytic neurotoxin related to TxP-I

A cDNA library established from polyadenylated RNA isolated from the predatory mite Pyemotes tritici was screened for cDNAs homologous to tox34, a cDNA encoding an insect-selective paralytic neurotoxin known as TxP-I. Most of the cDNA inserts of homologous clones were shorter or of equal length to t...

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Bibliographic Details
Published inToxicon (Oxford) Vol. 31; no. 3; p. 319
Main Authors Tomalski, M D, Hutchinson, K, Todd, J, Miller, L K
Format Journal Article
LanguageEnglish
Published England 01.03.1993
Subjects
Amino Acid Sequence
Animals
Baculoviridae - genetics
Base Sequence
Cloning, Molecular
DNA - genetics
Lethal Dose 50
Mites - genetics
Mites - metabolism
Molecular Sequence Data
Moths
Neurotoxins - biosynthesis
Neurotoxins - toxicity
Nucleic Acid Hybridization
Plasmids
Recombination, Genetic
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Summary:A cDNA library established from polyadenylated RNA isolated from the predatory mite Pyemotes tritici was screened for cDNAs homologous to tox34, a cDNA encoding an insect-selective paralytic neurotoxin known as TxP-I. Most of the cDNA inserts of homologous clones were shorter or of equal length to tox34 but a few were longer. Further investigation into the nature of these longer clones led to the identification, sequencing and expression of a distinct cDNA referred to as tox21A. This cDNA is predicted to encode a polypeptide which shares approximately 83% amino acid identity with TxP-I. Larvae infected with a recombinant baculovirus expressing tox21A are paralyzed during infection in a manner similar to larvae infected with tox34-expressing recombinants. The tox21A cDNA may represent a duplicated and diverged copy of the TxP-I gene.
ISSN:0041-0101
DOI:10.1016/0041-0101(93)90149-D