A rapid and sensitive micro-assay to determine the capacity of quinones to undergo redox cycling

• Published in: Toxicology letters Vol. 48; no. 2; p. 151
• Main Authors: Hart, L A, van der Wal, N A, Koster, A S, Labadie, R P
• Format: Journal Article
• Language: English
• Published: Netherlands 01.08.1989
• Subjects: Animals; Aziridines; Benzoquinones; Chromatography, Gas; Cyclohexenes; Erythrocytes - metabolism; Methemoglobin - biosynthesis; Microchemistry; Oxidation-Reduction; Oxyhemoglobins - metabolism; Quinones - blood; Quinones - metabolism; Sheep

Using a series of aziridinyl-benzoquinones it is shown that the conversion of oxyhemoglobin to methemoglobin in sheep erythrocytes is correlated with the capacity of each quinone to undergo redox cycling. Based on these findings a semiquantitative assay is developed for the rapid screening of redox...