Transformation of a Texas cotton cultivar by using Agrobacterium and the shoot apex

• Published in: Theoretical and applied genetics Vol. 98; no. 2; pp. 252 - 256
• Main Authors: Zapata, C, Park, S.H, El-Zik, K.M, Smith, R.H
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.02.1999; Berlin Springer Nature B.V
• Subjects: Agrobacterium tumefaciens; beta-glucuronidase; Biological and medical sciences; Biotechnology; Classical genetics, quantitative genetics, hybrids; Cotton; Cultivars; drug resistance; explants; Fundamental and applied biological sciences. Psychology; gene transfer; Genetic aspects; Genetic engineering; genetic markers; Genetic technics; Genetic transformation; Genetics of eukaryotes. Biological and molecular evolution; Gossypium hirsutum; Gram-negative bacteria; Health aspects; in vitro selection; kanamycin; kinases; Methods. Procedures. Technologies; neomycin phosphotransferase ii; Physiological aspects; Plant genetics; Pteridophyta, spermatophyta; regenerative ability; reporter genes; Seeds; shoot apices; Sucrose; Transgenic animals and transgenic plants; Transgenic plants; Vegetals; United States; United States > US; Texas; Malvaceae; Genetic transformation; Selection; Agrobacterium tumefaciens; Explant; Experimental protocol; Fiber crop; Regeneration; Dicotyledones; Angiospermae; Bacteria; Kanamycin; Spermatophyta; Gossypium hirsutum; Transgenic plant; Rhizobiaceae; Apex; Cultivar
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s001220051065

Transgenic cotton (Gossypium hirsutum L.) plants of a Texas cultivar CUBQHRPIS were obtained using Agrobacterium-mediated transformation coupled with the use of shoot-apex explants. After inoculation with A. tumefaciens strain LBA 4404 containing the pBI121 plasmid, regeneration of primary plants was carried out in a medium containing kanamycin (100 mg l-1). Progeny obtained by selfing were germinated in the greenhouse and selected for expression of the T-DNA marker gene encoding neomycin phosphotransferase II (NPTII) by painting kanamycin (2%) on the leaves. Plants that survived the leaf painting were analyzed by DNA blots. Evidence for integration of the transgene (GUS) was observed in two successive generations from the regenerants (T0). The transformed plants appeared to have more than one copy of the T-DNA.