Induction of Th1 polarized immune responses by thiolated Eudragit-coated F4 and F18 fimbriae of enterotoxigenic Escherichia coli

• Published in: European journal of pharmaceutics and biopharmaceutics Vol. 79; no. 2; pp. 226 - 231
• Main Authors: Lee, Won-Jung, Cha, Seungbin, Shin, Minkyoung, Islam, Mohammad Ariful, Cho, Chong-su, Yoo, Han Sang
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.10.2011; Elsevier
• Subjects: Animals; Antigens, Bacterial - immunology; Bacterial Vaccines - administration & dosage; Bacterial Vaccines - chemistry; Bacterial Vaccines - immunology; Biological and medical sciences; Cell Line; Enterotoxigenic Escherichia coli - immunology; Escherichia coli; Escherichia coli Infections - immunology; Escherichia coli Infections - prevention & control; Female; Fimbriae Proteins - immunology; Fimbriae, Bacterial - immunology; General pharmacology; Immune response; In vitro test; Interleukin; Interleukin-4 - immunology; Interleukin-6 - immunology; Intestinal Mucosa - immunology; Medical sciences; Mice; Mice, Inbred ICR; Microsphere; Microspheres; Nitric oxide; Nitric Oxide - immunology; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Polymethacrylic Acids - chemistry; Polymethacrylic Acids - pharmacology; Th1 Cells - immunology; Tumor Necrosis Factor-alpha - immunology; In vitro test; Microsphere; Immune response; Interleukin; Nitric oxide; Pharmaceutical technology; Induction; Escherichia coli; Thiolate; Cytokine; Th1 lymphocyte; In vitro; T-Lymphocyte; Bacteria; Microparticle; Enterobacteriaceae
• ISSN: 0939-6411; 1873-3441
• DOI: 10.1016/j.ejpb.2011.04.016

F4 or F18 loaded TEMS induces Th1 related immune responses in RAW 264.7 cells and mouse splenocytes. Diarrhea in newborn and weaned piglets is mainly induced by enterotoxigenic Escherichia coli (ETEC) with fimbriae F4 (K88) and F18 (F107). In this study, we evaluated F4 and F18 coated with thiolated Eudragit microspheres (TEMS) as a candidate for an oral vaccine. The average particle sizes of TEMS, F4-loaded TEMS, and F18-loaded TEMS were measured as 4.2 ± 0.75 μm, 4.7 ± 0.50 μm, and 4.5 ± 0.37 μm, respectively. F4 is more efficiently encapsulated than F18 in the loading with TEMS. In the release test, F4 and F18 fimbriae were protected in acidic circumstances, whereas most were released at pH 7.4 of intestine circumstances. Production of TNF-α and NO from RAW 264.7 cells was increased in a time-dependent manner after exposure to all groups, whereas only F4- or F18-loaded TEMS-stimulated IL-6 secretion. The levels of IFN-γ from mouse splenocytes after exposure to F4 or F18 were increased while IL-4 was not detectable. These results suggest that F4- and F18-loaded TEMS may effectively induce immune response with the efficient release of antigens to appropriate target sites.