Behaviour and occurrence of estrogens in municipal sewage treatment plants — II. Aerobic batch experiments with activated sludge

• Published in: The Science of the total environment Vol. 225; no. 1; pp. 91 - 99
• Main Authors: Ternes, T.A, Kreckel, P, Mueller, J
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 12.01.1999
• Subjects: Activated sludge; Aerobiosis; Batch experiments; Biodegradation, Environmental; Contraceptives; Contraceptives, Oral, Synthetic - analysis; Contraceptives, Oral, Synthetic - toxicity; Estradiol - analysis; Estradiol Congeners - analysis; Estradiol Congeners - toxicity; Estrogens; Estrogens - analysis; Estrogens - toxicity; Ethinyl Estradiol - analysis; Female; Germany; Glucuronates - analysis; Glucuronidase - analysis; Glucuronides; Humans; Mestranol - analysis; Sewage - adverse effects; Sewage - analysis; Sewage treatment plants; Germany; Activated sludge; Glucuronides; Contraceptives; Batch experiments; Sewage treatment plants; Estrogens
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/S0048-9697(98)00335-0

Aerobic batch experiments containing a diluted slurry of activated sludge from a real sewage treatment plant (STP) near Frankfurt/Main were undertaken, in order to investigate the persistence of natural estrogens and contraceptives under aerobic conditions. The batch experiments showed that while in contact with activated sludge the natural estrogen 17β-estradiol was oxidized to estrone, which was further eliminated in the batch experiments in an approximate linear time dependence. Further degradation products of estrone were not observed. 16α-hydroxyestrone was rapidly eliminated, again without detection of further degradation products. The contraceptive 17α-ethinylestradiol was principally persistent under the selected aerobic conditions, whereas mestranol was rapidly eliminated and small portions of 17α-ethinylestradiol were formed by demethylation. Additionally, two glucuronides of 17β-estradiol (17β-estradiol-17-glucuronide and 17β-estradiol-3-glucuronide) were cleaved in contact with the diluted activated sludge solution and thus 17β-estradiol was released. The glucuronidase activity of the activated sludge was further confirmed by the cleavage of 4-methylumbelliferyl-β- d-glucuronide (MUF-β-glucuronide) in a solution of a activated sludge slurry and Milli-Q-water (1:100, v/v). The turnover rate obtained was approximately steady state, with a turnover rate of 0.1 μmol/l for the released MUF. Hence, it is very likely that the glucuronic acid moiety of 17β-estradiol glucuronides and other estrogen glucuronides become cleaved in a real municipal STP, so that the concentrations of the free estrogens increase.