Relationship between plasma apolipoproteinB concentrations, apolipoproteinB/apolipoproeinA-I and HDL subclasses distribution

• Published in: Clinica chimica acta Vol. 388; no. 1; pp. 148 - 155
• Main Authors: Tian, Li, Wu, Xinwei, Fu, Mingde, Qin, Yang, Xu, Yanhua, Jia, Lianqun
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2008
• Subjects: ApoB100; ApoB100/apoA-I ratio; Apolipoprotein A-I - blood; Apolipoproteins B - blood; Cholesterol, HDL - blood; Cholesterol, HDL - classification; Coronary heart disease; Female; HDL subclasses distribution; Humans; Male; Middle Aged; Triglycerides - blood; ApoB100; ApoB100/apoA-I ratio; HDL subclasses distribution; Coronary heart disease
• ISSN: 0009-8981; 1873-3492
• DOI: 10.1016/j.cca.2007.10.028

To analyze the relationship between plasma apoB100 concentrations, apoB100/apoA-I ratio and the alteration of HDL subclasses distribution. The apoA-I contents of plasma HDL subclasses were quantitated by 2-dimensional gel electrophoresis coupled with immunodetection in 506 subjects. The subjects in the highest tertile of apoB100 groups had significantly higher small-sized preβ 1–HDL, however lower large-sized HDL 2a, HDL 2b than the subjects in the lowest tertile of apoB100 group. Furthermore, a significant down in the HDL 2b/preβ 1–HDL values (from 6.8 to 1.6) with a rise in apoB100/apoA-I ratio (from 0.4 to 1.4) were observed. Compared to subjects with apoB100/apoA-I ratio < 0.9, the subjects with apoB100/apoA-I ratio ≥ 0.9 had significantly higher small-sized preβ 1–HDL whereas lower HDL 3a, HDL 3b and large-sized HDL 2a, HDL 2b. Pearson correlation revealed that concentrations of apoB100 were positively correlated with preβ 1–HDL but negatively correlated with HDL 2a and HDL 2b, and in multivariate analysis, all HDL subclasses were independently associated with the apoB100/apoA-I ratio. The apoB100 concentrations, especially apoB100/apoA-I ratio could reflect sensitively the alteration of HDL subclasses distribution. And HDL subclasses distribution characteristics of hyperlipidemic subjects appeared in the subjects with apoB100/apoA-I ratio ≥ 0.9, which indicated the efficiency of RCT was weakened and the maturation of HDL was blocked.