A radiation hybrid map of 18 growth factor, growth factor receptor, hormone receptor, or neurotransmitter receptor genes on the distal region of the long arm of chromosome 5

• Published in: Genomics (San Diego, Calif.) Vol. 13; no. 3; pp. 803 - 808
• Main Authors: Warrington, J.A., Bailey, S.K., Armstrong, E., Aprelikova, O., Alitalo, K., Dolganov, G.M., Wilcox, A.S., Sikela, J.M., Wolfe, S.F., Lovett, M., Wasmuth, J.J.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.07.1992; Elsevier
• Subjects: Base Sequence; Biological and medical sciences; chromosome 5; Chromosome Mapping - methods; Chromosomes, Human, Pair 5; Classical genetics, quantitative genetics, hybrids; DNA - genetics; Fundamental and applied biological sciences. Psychology; gene mapping; genes; Genetic Markers; Genetics of eukaryotes. Biological and molecular evolution; growth factors; Growth Substances - genetics; hormones; Human; Humans; Hybrid Cells - radiation effects; man; Molecular Sequence Data; neurotransmitters; receptors; Receptors, Cell Surface - genetics; Receptors, Neurotransmitter - genetics; Genetic mapping; Human; Hormone; Hybrid cell; B5-Chromosome; DNA; Neuromediator; Cytokine; Molecular cloning; Growth factor; Biological receptor; Hormonal receptor
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1016/0888-7543(92)90156-M

The distal portion of the long arm of human chromosome 5 contains an impressive number of genes encoding growth factors, growth factor receptors, and hormone/neurotransmitter receptors. The order of and relative distance between 18 of these genes was determined by radiation hybrid mapping. There is only a single gap in a contiguous radiation map from 5q22-5q35. For this set of radiation hybrids, one map unit (centiray) corresponds to 20–50 kb of DNA. Close physical proximity for several pairs of loci was predicted by the map. Two sets of these were found to be contained in single YAC clones. The physical map produced by radiation hybrid mapping should prove useful in efforts to identify four disease genes that have been assigned to distal 5q by linkage studies.