Binding of antigen in the absence of histocompatibility proteins by arsonate-reactive T-cell clones

• Published in: Cell Vol. 36; no. 4; pp. 879 - 888
• Main Authors: Rao, Anjana, Ko, William Weng-Ping, Faas, Susan J., Cantor, Harvey
• Format: Journal Article
• Language: English
• Published: Cambridge, MA Elsevier Inc 01.01.1984; Cell Press
• Subjects: Analysis of the immune response. Humoral and cellular immunity; Animals; Antigens - immunology; Azo Compounds - pharmacology; Binding Sites; Biological and medical sciences; Cells, Cultured; Clone Cells; DNA Replication - drug effects; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Haptens; histocompatibility antigen HLA; Histocompatibility Antigens - immunology; Immunobiology; Kinetics; Ligands; Lymphocyte Activation; lymphocytes T; Mice; Mice, Inbred Strains; Organs and cells involved in the immune response; p-azobenzenearsonate; p-Azobenzenearsonate - pharmacology; receptors; T-Lymphocytes - drug effects; T-Lymphocytes - immunology; Lymphoid cell; Rodentia; Activation; Cellular immunity; Binding site; Mechanism; Antigen; Vertebrata; Specificity; Mammalia; Investigation method; Mouse; Animal; T-Lymphocyte
• ISSN: 0092-8674; 1097-4172
• DOI: 10.1016/0092-8674(84)90037-0

Inducer T-cell clones reactive to the p-azobenzenearsonate (arsonate) hapten possess binding sites for radioactive arsanylated proteins, which are not present on clones with other antigen specificities. Binding occurred in the absence of histocompatibility proteins. Binding was specific for the p-azobenzenearsonate hapten, since unconjugated proteins and proteins conjugated to the nonactivating o-azobenzenearsonate hapten neither bound to the clones nor competed binding of radioactive antigen. One of the clones was studied in more detail, using a panel of structural analogs of arsonate conjugated to the carrier protein ovalbumin. All conjugates that activated the clone in the presence of antigen-presenting cells also competed binding of radioactive antigen in the absence of antigen-presenting cells. Nonactivating conjugates did not compete binding. Based on evidence in this and the succeeding paper (Rao et al., accompanying paper), we suggest that these arsonate-binding sites may include the physiological antigen receptors of arsonate-reactive Tcell clones.