TRPC1 is required for survival and proliferation of cochlear spiral ganglion stem/progenitor cells

Abstract Objective The present studies were designed to test the hypothesis that canonical transient receptor potential channel 1 (TRPC1) is required for the proliferation of cochlear spiral ganglion stem/progenitor cells (SPCs). Methods and materials TRPC1 were detected and evaluated in postnatal d...

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Published inInternational journal of pediatric otorhinolaryngology Vol. 79; no. 12; pp. 2290 - 2294
Main Authors Chen, Hsin-Chien, Wang, Chih-Hung, Shih, Cheng-Ping, Chueh, Sheau-Huei, Liu, Shu-Fan, Chen, Hang-Kang, Lin, Yi-Chun
Format Journal Article
LanguageEnglish
Published Ireland Elsevier B.V 01.12.2015
Subjects
Animals
Animals, Newborn
Canonical transient receptor potential channel
Cell Proliferation - physiology
Cell Survival - physiology
Cochlea
Cochlea - cytology
Gene Silencing
Mice, Inbred CBA
Otolaryngology
Pediatrics
Proliferation
Reverse Transcriptase Polymerase Chain Reaction
Spiral Ganglion - cytology
Spiral ganglion neuron
Stem Cells - physiology
Stem/progenitor cell
TRPC Cation Channels - genetics
TRPC Cation Channels - physiology
Spiral ganglion neuron
Cochlea
Stem/progenitor cell
Proliferation
Canonical transient receptor potential channel
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Summary:Abstract Objective The present studies were designed to test the hypothesis that canonical transient receptor potential channel 1 (TRPC1) is required for the proliferation of cochlear spiral ganglion stem/progenitor cells (SPCs). Methods and materials TRPC1 were detected and evaluated in postnatal day 1 CBA/CaJ mice pups derived-cochlear spiral ganglion SPCs by reverse transcription-polymerase chain reaction, Western blot, immunocytochemistry, and calcium imaging. The cell viability and proliferation of the spiral ganglion SPCs following si-RNA mediated knockdown of TRPC1 or addition of TRPC channel blocker SKF9635 were compared to controls. Results In spiral ganglion SPCs, TRPC1 was found to be the most abundantly expressed TRPC subunit and shown to contribute to store-operated calcium entry. Silencing of TRPC1 or addition of TRPC channel blockers significantly decreased the rate of cell proliferation. Conclusion The results suggest that TRPC1 might serve as an essential molecule in regulating the proliferation of spiral ganglion SPCs.
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ISSN:0165-5876
1872-8464
DOI:10.1016/j.ijporl.2015.10.027