A one-step enzyme-linked immunosorbent assay to detect anti-CMV antibodies; development and clinical validation

• Published in: Journal of virological methods Vol. 17; no. 1; pp. 141 - 148
• Main Authors: Wielaard, F., Scherders, J., Dagelinckx, C., Gausset, P.
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 01.08.1987; Amsterdam Elsevier; New York, NY
• Subjects: Antibodies, Viral - analysis; Antibody; Antigens, Viral - immunology; Biological and medical sciences; Blood Donors; Cytomegalovirus; Cytomegalovirus - immunology; ELISA; Enzyme-Linked Immunosorbent Assay; Fundamental and applied biological sciences. Psychology; Humans; Latex Fixation Tests; Microbiology; Predictive Value of Tests; Screening test; Techniques used in virology; Virology; Cytomegalovirus; Screening test; Antibody; ELISA; Virus; Herpesviridae; Serum; Enzyme immunoassay; Betaherpesvirinae; ELISA assay; Immunological method
• ISSN: 0166-0934; 1879-0984
• DOI: 10.1016/0166-0934(87)90077-2

A simple one-step ELISA to detect anti-CMV antibodies was developed. The test was based on the inhibition principle, and used anti-CMV coated microtitre plates, CMV nuclear antigen and anti-CMV Fab'-HRP conjugate; total assay time was 1.5 h. The use of Fab'-HRP conjugates improved discrimination between positive and negative sera as compared with IgG-HRP conjugates. In an in-house clinical study, the one-step ELISA showed a 98.4% correlation with the latex agglutination test. The test could be demonstrated to be suitable for both serum and citrate- or EDTA-plasma specimens; heparin-plasma gave somewhat higher absorbance values. In an external clinical validation, the one-step ELISA showed a 99.5% correlation with the latex agglutination test, and was found to be highly suitable for screening of blood donor specimens in a blood bank setting.