Coordinate control of growth arrest states in normal human diploid fibroblasts: Effect of cloned SV40 tumor antigen genes

• Published in: Experimental cell research Vol. 184; no. 2; pp. 297 - 303
• Main Authors: Donahue, Laurel M., Stein, Gretchen H.
• Format: Journal Article
• Language: English
• Published: Orlando, FL Elsevier Inc 01.10.1989; Elsevier
• Subjects: Ageing, cell death; Antigens, Polyomavirus Transforming - genetics; Antigens, Polyomavirus Transforming - metabolism; Biological and medical sciences; Cell Division; Cell physiology; Clone Cells - metabolism; Diploidy; Fibroblasts - cytology; Fibroblasts - metabolism; Fibroblasts - physiology; Fundamental and applied biological sciences. Psychology; Gene Expression; Humans; Molecular and cellular biology; Phenotype; Transfection; Chromosomal aberration; Human; Senescence; Radiolabelling; Polyomavirus; Aneuploidy; Molecular interaction; Papovaviridae; Quiescence; Virus; Phenotype; Regulation(control); Transfection; Gene; Molecular cloning; SV40 virus; Fibroblast
• ISSN: 0014-4827; 1090-2422
• DOI: 10.1016/0014-4827(89)90329-7

The growth arrest states of quiescence and senescence in normal human diploid fibroblasts (HDF) are related but distinct phenomena. In an effort to (1) understand the extent to which arrest in the quiescent state and arrest in the senescent state share common regulatory mechanisms, and (2) test our hypothesis that when the quiescent phenotype is altered in HDF, then the senescent phenotype is likewise altered, we have transfected HDF with cloned SV40 tumor antigen genes. Introduction of the tumor antigen genes results in a loss of the ability to enter both the quiescent arrest state and the senescent arrest state but does not immortalize the cells or create significant aneuploidy.