A Murine Herpesvirus Closely Related to Ubiquitous Human Herpesviruses Causes T-Cell Depletion

• Published in: Journal of virology Vol. 91; no. 9
• Main Authors: Patel, Swapneel J, Zhao, Guoyan, Penna, Vinay R, Park, Eugene, Lauron, Elvin J, Harvey, Ian B, Beatty, Wandy L, Plougastel-Douglas, Beatrice, Poursine-Laurent, Jennifer, Fremont, Daved H, Wang, David, Yokoyama, Wayne M
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.05.2017
• Subjects: Animals; Base Sequence; Betaherpesvirinae; CD4-Positive T-Lymphocytes - cytology; CD4-Positive T-Lymphocytes - immunology; CD8-Positive T-Lymphocytes - cytology; CD8-Positive T-Lymphocytes - immunology; Disease Models, Animal; DNA, Viral - genetics; Genetic Diversity and Evolution; Genome, Viral - genetics; Herpesviridae; Herpesviridae - classification; Herpesvirus 6, Human - genetics; Herpesvirus 7, Human - genetics; Humans; Immune Evasion - genetics; Immune Evasion - immunology; Lymphocyte Count; Lymphocyte Depletion; Mice; Mice, Inbred BALB C; Open Reading Frames - genetics; Phylogeny; Roseolovirus; Roseolovirus Infections - virology; Sequence Analysis, DNA; Thymus Gland - virology; herpesviruses; roseolovirus
• ISSN: 0022-538X; 1098-5514
• DOI: 10.1128/JVI.02463-16

The human roseoloviruses human herpesvirus 6A (HHV-6A), HHV-6B, and HHV-7 comprise the genus of the human subfamily. Infections with these viruses have been implicated in many diseases; however, it has been challenging to establish infections with roseoloviruses as direct drivers of pathology, because they are nearly ubiquitous and display species-specific tropism. Furthermore, controlled study of infection has been hampered by the lack of experimental models, and until now, a mouse roseolovirus has not been identified. Herein we describe a virus that causes severe thymic necrosis in neonatal mice, characterized by a loss of CD4 T cells. These phenotypes resemble those caused by the previously described mouse thymic virus (MTV), a putative herpesvirus that has not been molecularly characterized. By next-generation sequencing of infected tissue homogenates, we assembled a contiguous 174-kb genome sequence containing 128 unique predicted open reading frames (ORFs), many of which were most closely related to herpesvirus genes. Moreover, the structure of the virus genome and phylogenetic analysis of multiple genes strongly suggested that this virus is a betaherpesvirus more closely related to the roseoloviruses, HHV-6A, HHV-6B, and HHV-7, than to another murine betaherpesvirus, mouse cytomegalovirus (MCMV). As such, we have named this virus murine roseolovirus (MRV) because these data strongly suggest that MRV is a mouse homolog of HHV-6A, HHV-6B, and HHV-7. Herein we describe the complete genome sequence of a novel murine herpesvirus. By sequence and phylogenetic analyses, we show that it is a betaherpesvirus most closely related to the roseoloviruses, human herpesviruses 6A, 6B, and 7. These data combined with physiological similarities with human roseoloviruses collectively suggest that this virus is a murine roseolovirus (MRV), the first definitively described rodent roseolovirus, to our knowledge. Many biological and clinical ramifications of roseolovirus infection in humans have been hypothesized, but studies showing definitive causative relationships between infection and disease susceptibility are lacking. Here we show that MRV infects the thymus and causes T-cell depletion, suggesting that other roseoloviruses may have similar properties.