Insulin in the vitreous of the normal and streptozotocin-induced diabetic rat
Insulin has been detected by ELISA in the vitreous of the normal and streptozotocindiabetic rat at levels for both about 1% of those in serum. 131I-labeled insulin, administered to conscious rats via an indwelling cannula in the right atrium, was found to cross the blood-ocular barrier into the vitr...
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Published in | Peptides (New York, N.Y. : 1980) Vol. 13; no. 4; pp. 671 - 675 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
New York, NY
Elsevier Inc
01.07.1992
Elsevier Science |
Subjects | |
Online Access | Get full text |
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Summary: | Insulin has been detected by ELISA in the vitreous of the normal and streptozotocindiabetic rat at levels for both about 1% of those in serum.
131I-labeled insulin, administered to conscious rats via an indwelling cannula in the right atrium, was found to cross the blood-ocular barrier into the vitreous. Autoradiographic gel analysis showed the peptide was transferred as an intact molecule. Vitreous insulin levels reflected serum levels as seen in relatively constant vitreous-to-serum insulin ratios over a wide range of serum insulin concentrations. The rate of blood-to-vitreous passage of insulin was about the same in normal as in diabetic rats (
fasting serum glucose≥21
mM
). At least a portion of vitreous insulin is therefore of pancreatic origin, and retinal tissue in the normal and diabetic animal is thus accessible to circulating hormone. The blood-ocular barrier is unaltered in streptozotocin diabetes with regard to insulin passage. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0196-9781 1873-5169 |
DOI: | 10.1016/0196-9781(92)90171-X |