Characterization of polysaccharide fractions from fruit of Actinidia arguta and assessment of their antioxidant and antiglycated activities

• Published in: Carbohydrate polymers Vol. 210; pp. 73 - 84
• Main Authors: Zhu, Rugang, Zhang, Xinyu, Wang, Yu, Zhang, Lijiao, Zhao, Jing, Chen, Gang, Fan, Jungang, Jia, Youfeng, Yan, Fengwen, Ning, Chong
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 15.04.2019
• Subjects: Actinidia - chemistry; Actinidia argute; Animals; Antiglycation; Antioxidants - chemistry; Antioxidants - pharmacology; Antioxidation; Cattle; Characterization; Fruit - chemistry; Glycosylation - drug effects; Lipid Peroxidation - drug effects; Polysaccharide; Polysaccharides - chemistry; Polysaccharides - pharmacology; Serum Albumin, Bovine - metabolism; NBT; CML; DTNB; AGEs; CEL; BHT; Characterization; Antiglycation; RG; SPS; WPS; Antioxidation; AAPs; Actinidia argute; Polysaccharide; HG
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2019.01.037

•Polysaccharides (AAPs) were extracted from the fruits of Actinidia argute.•AAPs were separated into four parts, including WPS, SPS-1, SPS-2 and SPS-3.•SPS-3, SPS-2 and SPS-1 were pectin and WPS was starch-like polysaccharides.•SPS-3 displayed the strongest antioxidant and antiglycated activities.•The antiglycated effects of AAPs may be of concern to their antioxidant activity. A novel cell-wall polysaccharides (AAPs) were extracted from the fruits of Actinidia arguta and separated into four parts which were named water-eluted polysaccharide (WPS), salt-eluted polysaccharide (SPS)-1, SPS-2 and SPS-3. The monosaccharide composition and structural analysis showed that SPS-3 and SPS-2 were homogalacturonan (HG)-rich pectin, SPS-1 was rhamnogalacturonan (RG)-rich pectin and WPS was starch-like polysaccharides. All four kinds of polysaccharides displayed the ability to scavenge free radicals, chelate iron ion, inhibit lipid peroxidation and inhibit protein glycation, but SPS was obviously stronger than WPS. Especially SPS-3 displayed the strongest antioxidant and anti-glycated activities. In addition, the inhibitory effect of AAPs on AGEs formation is attributed to the inhibitory activity on the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds, suggesting that its mechanisms of antiglycated effects may be of concern to their antioxidant activities.