Screening DNA-targeted anticancer drug in vitro based on the drug-conjugated DNA by resonance light scattering technique

• Published in: Biosensors & bioelectronics Vol. 25; no. 8; pp. 1947 - 1952
• Main Authors: Chen, Zhanguang, Song, Tianhe, Wang, Shaobin, Chen, Xi, Chen, Junhui, Li, Yuqing
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 15.04.2010; Elsevier
• Subjects: Animals; Anthracycline drug; Anticancer; Antineoplastic Agents - analysis; Antineoplastic Agents - chemistry; Biological and medical sciences; Biosensing Techniques - methods; Biotechnology; DNA, Neoplasm - analysis; DNA, Neoplasm - chemistry; DNA-targeted; Drug Delivery Systems - methods; Drug Evaluation, Preclinical - methods; Drug screening; Fundamental and applied biological sciences. Psychology; Humans; Refractometry - methods; Reproducibility of Results; Resonance light scattering; Sensitivity and Specificity; Spectrometry, Fluorescence - methods; Drug screening; Anticancer; DNA-targeted; Anthracycline drug; Resonance light scattering; Antineoplastic agent; Drug; Targeting; Light scattering; In vitro; Screening; Target; DNA; Anthracyclins; Resonance; Technique
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2010.01.011

A sensitive and reliable assay has been developed to directly screen DNA-targeted anticancer drugs in vitro via using resonance light scattering (RLS) technique. The results of experiments displayed that the increment of RLS intensity was directly proportional to the antitumor effect of anticancer drugs. Through the RLS spectra, the activities of four drugs have been demonstrated as mitoxantrone (MIT) > epirarubicin (EPI) > daunorubicin (DAU) > adriamycin (ADM). However, to further verify the activities of the above four drugs, binding constant ( k) for each of them has been calculated by RLS technique as follows: k RLS (MIT, 8.75 × 10 5 L mol −1) > k RLS (EPI, 6.58 × 10 5 L mol −1) > k RLS (DAU, 4.79 × 10 5 L mol −1) > k RLS (ADM, 3.82 × 10 5 L mol −1). Also, this RLS assay result was validated by seasoned vitro screening methods for anticancer drugs. In all, the proposed RLS is not only a simple, sensitive, objective and straightforward method, but also it is an unprecedented assay for primarily screening DNA-targeted anticancer drugs.