Knockout of cGAS and STING Rescues Virus Infection of Plasmid DNA-Transfected Cells

• Published in: Journal of virology Vol. 89; no. 21; pp. 11169 - 11173
• Main Authors: Langereis, Martijn A, Rabouw, Huib H, Holwerda, Melle, Visser, Linda J, van Kuppeveld, Frank J M
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.11.2015
• Subjects: Cellular Response to Infection; Coxsackievirus Infections - metabolism; CRISPR-Cas Systems; Cytosol - metabolism; Flow Cytometry; Gene Knockout Techniques; Gene Transfer Techniques; Green Fluorescent Proteins; HeLa Cells; Humans; Luminescent Proteins; Membrane Proteins - genetics; Membrane Proteins - metabolism; Mengovirus - metabolism; Nucleotidyltransferases - genetics; Nucleotidyltransferases - metabolism; Plasmids - genetics; Plasmids - metabolism; Red Fluorescent Protein; Signal Transduction - genetics; Signal Transduction - physiology; Spotlight; Transfection - methods

It is well known that plasmid DNA transfection, prior to virus infection, negatively affects infection efficiency. Here, we show that cytosolic plasmid DNA activates the cGAS/STING signaling pathway, which ultimately leads to the induction of an antiviral state of the cells. Using a transient one-pl...