Knockout of cGAS and STING Rescues Virus Infection of Plasmid DNA-Transfected Cells

It is well known that plasmid DNA transfection, prior to virus infection, negatively affects infection efficiency. Here, we show that cytosolic plasmid DNA activates the cGAS/STING signaling pathway, which ultimately leads to the induction of an antiviral state of the cells. Using a transient one-pl...

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Published inJournal of virology Vol. 89; no. 21; pp. 11169 - 11173
Main Authors Langereis, Martijn A, Rabouw, Huib H, Holwerda, Melle, Visser, Linda J, van Kuppeveld, Frank J M
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.11.2015
Subjects
Cellular Response to Infection
Coxsackievirus Infections - metabolism
CRISPR-Cas Systems
Cytosol - metabolism
Flow Cytometry
Gene Knockout Techniques
Gene Transfer Techniques
Green Fluorescent Proteins
HeLa Cells
Humans
Luminescent Proteins
Membrane Proteins - genetics
Membrane Proteins - metabolism
Mengovirus - metabolism
Nucleotidyltransferases - genetics
Nucleotidyltransferases - metabolism
Plasmids - genetics
Plasmids - metabolism
Red Fluorescent Protein
Signal Transduction - genetics
Signal Transduction - physiology
Spotlight
Transfection - methods
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Summary:It is well known that plasmid DNA transfection, prior to virus infection, negatively affects infection efficiency. Here, we show that cytosolic plasmid DNA activates the cGAS/STING signaling pathway, which ultimately leads to the induction of an antiviral state of the cells. Using a transient one-plasmid clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system, we generated cGAS/STING-knockout cells and show that these cells can be infected after plasmid DNA transfection as efficiently as nontransfected cells.
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Citation Langereis MA, Rabouw HH, Holwerda M, Visser LJ, van Kuppeveld FJM. 2015. Knockout of cGAS and STING rescues virus infection of plasmid DNA-transfected cells. J Virol 89:11169–11173. doi:10.1128/JVI.01781-15.
ISSN:0022-538X
1098-5514
DOI:10.1128/JVI.01781-15