Neurotransmitter-lymphocyte interactions: dual receptor modulation of lymphocyte proliferation and cAMP production

• Published in: Journal of neuroimmunology Vol. 24; no. 1; pp. 155 - 162
• Main Authors: Carlson, Sonia L., Brooks, William H., Roszman, Thomas L.
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 01.09.1989; Amsterdam Elsevier; New York, NY
• Subjects: Biological and medical sciences; Cyclic AMP; Cyclic AMP - biosynthesis; Dose-Response Relationship, Immunologic; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; Immunobiology; Ionomycin - pharmacology; Isoproterenol; Isoproterenol - immunology; Isoproterenol - pharmacology; Lymphocyte Activation - immunology; Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation; Mitogen; Neurotransmitter Agents - immunology; Neurotransmitter Agents - metabolism; Phytohemagglutinins; Receptors, Adrenergic, beta - immunology; T cell; T-Lymphocytes - immunology; T-Lymphocytes - metabolism; Tetradecanoylphorbol Acetate - pharmacology; Transmembrane signal; β-Adrenergic receptor; Cyclic AMP; Mitogen; Isoproterenol; T cell; Transmembrane signal; β-Adrenergic receptor; Cell proliferation; Agonist; β-»Adrenergic receptor; Molecular interaction; Biosynthesis; Monoclonal antibody; Cell surface; Modulation; Radioimmunoassay; T-Lymphocyte; Neurotransmitter; Lymphocyte
• ISSN: 0165-5728; 1872-8421
• DOI: 10.1016/0165-5728(89)90109-4

Stimulation of the β-adrenergic receptor on lymphocytes can decrease the proliferative response of these cells to mitogens We have found that simultaneous stimulation of T cells with the β-adrenergic agonist isoprotenenol and mitogens (phytohemagglutinin (PHA) and OKT3 monoclonal antibody) results in a 2- to 4-fold increase in cAMP production compared to cells exposed to isoproterenol alone. Mitogens alone have little effect on cAMP synthesis, but do activate the phosphatidylinositol (PI) cycle, suggesting that interactions may be occurring between the second messenger systems resulting in a cAMP synergy. Further experiments suggest that calcium may be involved in inducing the cAMP synergy observed in T cells. It is proposed that the synergy between β-adrenergic and mitogenic stimulation of T cells for cAMP may be involved in the mechanism of catecholamine modulation of lymphocyte function.