Characterisation of oligosaccharides from Drosophila melanogaster glycoproteins

• Published in: Biochimica et biophysica acta Vol. 1075; no. 2; pp. 146 - 153
• Main Authors: Williams, Phillip J., Wormald, Mark R., Dwek, Raymond A., Rademacher, Thomas W., Parker, George F., Roberts, David R.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 10.10.1991; Elsevier
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Carbohydrate Conformation; Carbohydrate Sequence; carbohydrate structure; Carbohydrates; Characterization; chemical analysis; chemistry; D. melanogaster; Drosophila melanogaster; Drosophila melanogaster - chemistry; Fundamental and applied biological sciences. Psychology; Glycoprotein; glycoproteins; Glycosylation; isolation & purification; larvae; Mannose; Mannose - chemistry; Mannose - isolation & purification; Membrane Glycoproteins; Membrane Glycoproteins - chemistry; Membrane Glycoproteins - isolation & purification; Molecular Sequence Data; Oligosaccharide; oligosaccharides; Oligosaccharides - chemistry; Oligosaccharides - isolation & purification; Oses; Other biological molecules; Characterization; Fuc; Oligosaccharide; Glycoprotein; HRP; GlcNac-ol N-acetylglucosaminitol; LSP2; GlcNAc; Xyl; Man; D. melanogaster; g.u; Larva; Arthropoda; Insecta; Glycoproteins; Invertebrata; Drosophila melanogaster; Diptera; Drosophilidae; Structural analysis
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/0304-4165(91)90245-C

An analysis of the released oligosaccharides from a membrane glycoprotein preparation of third instar larvae (3rdIL), and purified larval serum protein 2 (LSP2) from Drosophila melanogaster was performed. Sequential exoglycosidase digestion in combination with high-resolution gel permeation chromatography and partial acetolysis indicated the presence of two series of oligomannosides; one of these series was unusual and characterized by the presence of a core α1–6 linked fucose, the other was a typical mammalian oligomannose series containing the following isomers −D 1, −D 2, −D 12, −D 123 and −CD 123 as well as the unprocessed Man 9GlcNAc 2 structure. Conventional oligomannose could only be detected in the LSP2 sample. This study opens the way to use powerful molecular and classical genetic techniques to analyse the control and functional significance of glycosylation in higher organisms.