Ameliorative effect of chromium- d -phenylalanine complex on indomethacin-induced inflammatory bowel disease in rats

• Published in: Biomedicine & pharmacotherapy Vol. 89; pp. 1061 - 1066
• Main Authors: Nagarjun, S, Dhadde, Shivsharan B, Veerapur, Veeresh P, Thippeswamy, B.S, Chandakavathe, Baburao N
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.05.2017
• Subjects: Animals; Anti-inflammatory; Anti-Inflammatory Agents, Non-Steroidal - toxicity; Antioxidant; Chromium Compounds - chemistry; Chromium Compounds - pharmacology; Glutathione; Indomethacin - toxicity; Inflammatory bowel disease; Inflammatory Bowel Diseases - chemically induced; Internal Medicine; Lipid Peroxidation; Medical Education; Myeloperoxidase; Peroxidase - metabolism; Phenylalanine - chemistry; Phenylalanine - pharmacology; Rats; Rats, Wistar; Sulfasalazine - pharmacology; Inflammatory bowel disease; Myeloperoxidase; Antioxidant; Anti-inflammatory
• ISSN: 0753-3322; 1950-6007
• DOI: 10.1016/j.biopha.2017.02.042

Abstract Present study was designed to evaluate the effect of chromium- d -phenylalanine complex (Cr ( d -phe)3 ) on indomethacin-induced inflammatory bowel disease (IBD) in rats. Adult Wistar rats were pretreated with vehicle/Cr ( d -phe)3 (30, 60 and 90 μg/kg, p.o.) for 11 days. On day 8 and 9, after one h of the above mentioned treatment, indomethacin (7.5 mg/kg/day, s.c.) was administered to induce IBD. On day 12, blood samples were collected from animals for lactate dehydrogenase (LDH) estimation and ileum was isolated for macroscopic scoring, biochemical estimation (lipid peroxidation, reduced glutathione and myeloperoxidase activity) and histopathological study. Administration of indomethacin significantly altered the serum LDH, macroscopic and microscopic appearance and biochemical parameters in ileum tissue. Cr ( d -phe)3 , at all the tested doses, caused a significant reversal of changes induced by indomethacin. Present study demonstrates the protective effect of Cr ( d -phe)3 against indomethacin-induced IBD in rats. The observed protective effect might be attributed to the antioxidant and anti-inflammatory properties of Cr ( d -phe)3.