Lack of effect of acute acetaldehyde treatment on X chromosome segregation in Drosophila melanogaster females

• Published in: Mutation Research Vol. 320; no. 1; pp. 1 - 7
• Main Authors: Rey, Mónica, Palermo, Ana M., Muñoz, Enzo R.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 1994; Elsevier
• Subjects: Acetaldehyde; Acetaldehyde - toxicity; Alcohol Dehydrogenase - metabolism; Aneuploidy; Animals; Biological and medical sciences; Chemical mutagenesis; Chi-Square Distribution; Dose-Response Relationship, Drug; Drosophila melanogaster; Drosophila melanogaster - drug effects; Drosophila melanogaster - enzymology; Drosophila melanogaster - genetics; Ethanol - toxicity; Female; Male; Medical sciences; Meiosis - drug effects; Mutagenicity Tests; Nondisjunction; Nondisjunction, Genetic; Toxicology; X Chromosome - drug effects; X chromosome segregation; X chromosome segregation; Aneuploidy; Nondisjunction; Drosophila melanogaster; Acetaldehyde; Chromosomal aberration; Segregation; Mutagenicity testing; Insecta; Drosophilidae; Arthropoda; Female; X-Chromosome; Invertebrata; Diptera
• ISSN: 0165-1218; 0027-5107
• DOI: 10.1016/0165-1218(94)90054-X

The effect of acute acetaldehyde treatments on X chromosome segregation was tested in germinal cells of Drosophila melanogaster females. The experiments were carried out using a test system where the nondisjunctional females (XXY) and only 1/4 of the expected regular progeny are viable. 24 h old virgin females were exposed for 60 min to 3, 4 and 5% acetaldehyde solutions by means of soaked tissue paper placed at the bottom of regular culture vials. After mating the females were brooded daily. Two additional experiments were performed with 0–2 h old and 4–5 day old virgin females using a 4% acetaldehyde solution. The results obtained show that acetaldehyde did not affect X chromosomal segregation in oocytes. This lack of effect could result from the highly efficient ADH-ALDH dependent detoxifying mechanism operating in Drosophila melanogaster.