Autoradiographic localization of receptors for neuropeptide FF, FLFQPQRFamide, in human spinal sensory system

The regional distribution of FLFQPQRFamide binding sites on fresh unfixed cryostata sections from post mortem specimens of human spinal cord and lower medulla oblongata was studied by quantitative autoradiographic methods using [ 125I]YLFQPQRFamide as ligand. Samples were taken from fives cases who...

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Published inBrain research Vol. 633; no. 1; pp. 127 - 132
Main Authors Allard, M., Jordan, D., Zajac, J.M., Ries, C., Martin, D., Monkouanga, D., Kopp, N., Simonnet, G.
Format Journal Article
LanguageEnglish
Published London Elsevier B.V 07.01.1994
Amsterdam Elsevier
New York, NY
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Summary:The regional distribution of FLFQPQRFamide binding sites on fresh unfixed cryostata sections from post mortem specimens of human spinal cord and lower medulla oblongata was studied by quantitative autoradiographic methods using [ 125I]YLFQPQRFamide as ligand. Samples were taken from fives cases who had died with no history of neurological disease at ages ranging from 5 months to 66 years. The biochemical and pharmacological characteristics of [ 125I]YLFQPQRFamide binding to mounted tissue sections were comparable to those reported for the rat in a previous study. [ 125I]YLFQPQRFamide appeared to interact reversibly with high affinity binding sites ( K d = 0.06nM ), distinct from opiate receptors. Sites labelled with [ 125I]YLFQPQRFamide were distributed unevenly within the human spinal cord and lower mmedulla oblongata, with the highest density in the superficial layers of the dorsal horn and the spinal trigeminal nucleus. Although moderate labelling was observed in the ventral part of spinal grey matter, dense labelling appeared in the gracile and cuneate nuclei. No binding sites were detected in white matter. These results show that, as in the rat, FLFQPQRFamide receptors in the human spinal cord and lower medulla oblongata, are mainly concentrated within spinal areas implicated in the analgesic action of opiates. The possible role of these receptors in modulating spinal nociceptive information is discussed with respect to the pharmacological effects of substances acting on FLFQPQRFamide receptors in animals.
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ISSN:0006-8993
1872-6240
DOI:10.1016/0006-8993(94)91531-8