Characteristics of lung resistance and elastance associated with tracheal stenosis and intrapulmonary airway narrowing in ex vivo sheep lungs

• Published in: Respiratory research Vol. 25; no. 1; pp. 332 - 14
• Main Authors: Yasuda, Yuto, Maksym, Geoffrey N, Wang, Lu, Chitano, Pasquale, Seow, Chun Y
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 09.09.2024; BioMed Central; BMC
• Subjects: Air flow; Airway Resistance - physiology; Airway smooth muscle; Animals; Central airway obstruction; Disease Models, Animal; Elasticity; Health aspects; In Vitro Techniques; Lung - physiopathology; Lung mechanics; Peripheral airway narrowing; Sheep; Stenosis; Trachea; Tracheal Stenosis - physiopathology; Transpulmonary pressure; Ventilation frequency; Canada; Peripheral airway narrowing; Transpulmonary pressure; Airway smooth muscle; Ventilation frequency; Central airway obstruction; Lung mechanics
• ISSN: 1465-993X; 1465-9921; 1465-993X
• DOI: 10.1186/s12931-024-02959-z

Understanding the characteristics of pulmonary resistance and elastance in relation to the location of airway narrowing, e.g., tracheal stenosis vs. intrapulmonary airway obstruction, will help us understand lung function characteristics and mechanisms related to different airway diseases. In this study, we used ex vivo sheep lungs as a model to measure lung resistance and elastance across a range of transpulmonary pressures (5-30 cmH O) and ventilation frequencies (0.125-2 Hz). We established two tracheal stenosis models by inserting plastic tubes into the tracheas, representing mild (71.8% lumen area reduction) and severe (92.1%) obstructions. For intrapulmonary airway obstruction, we induced airway narrowing by challenging the lung with acetylcholine (ACh). We found a pattern change in the lung resistance and apparent lung elastance as functions of ventilation frequency that depended on the transpulmonary pressure (or lung volume). At a transpulmonary pressure of 10 cmH O, lung resistance increased with ventilation frequency in severe tracheal stenosis, whereas in ACh-induced airway narrowing the opposite occurred. Furthermore, apparent lung elastance at 10 cmH O decreased with increasing ventilation frequency in severe tracheal stenosis whereas in ACh-induced airway narrowing the opposite occurred. Flow-volume analysis revealed that the flow amplitude was much sensitive to ventilation frequency in tracheal stenosis than it was in ACh induced airway constriction. Results from this study suggest that lung resistance and apparent elastance measured at 10 cmH O over the frequency range of 0.125-2 Hz can differentiate tracheal stenosis vs. intrapulmonary airway narrowing in ex vivo sheep lungs.