A protective monoclonal antibody with dual specificity for Plasmodium falciparum and Plasmodium berghei circumsporozoite proteins

• Published in: Experimental parasitology Vol. 74; no. 4; pp. 431 - 440
• Main Authors: Sina, Barbara J., Wright, Craig, Ballou, Ripley, Hollingdale, Michael
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.06.1992; Elsevier
• Subjects: Animals; Antibodies, Monoclonal - immunology; Antibodies, Monoclonal - isolation & purification; Antibodies, Protozoan - immunology; Antibody Specificity; Antigens, Protozoan - immunology; Biological and medical sciences; Blotting, Western; Circumsporozoite protein; Epitopes - immunology; Experimental protozoal diseases and models; Immunoglobulin M - immunology; Infectious diseases; Malaria; Malaria - immunology; Medical sciences; Mice; Mice, Inbred BALB C; Monoclonal antibodies; Parasitic diseases; Plasmodium berghei; Plasmodium berghei - immunology; Plasmodium falciparum; Plasmodium falciparum - immunology; Precipitin Tests; Protozoal diseases; Protozoan Proteins; Sporozoite immunity; Tropical medicine; Tumor Cells, Cultured; CS; PBS; Plasmodium falciparum; BSA; Plasmodium berghei; SDS; Malaria; IFA; Monoclonal antibodies; Sporozoite immunity; Circumsporozoite protein; ELISA; Protozoa; Protozoal disease; Immunization; Parasite; Sporozoit; Monoclonal antibody; Immunity; Biological activity; Immunological method; Infection; Proteins; Electrophoresis; Sporozoa
• ISSN: 0014-4894; 1090-2449
• DOI: 10.1016/0014-4894(92)90205-O

An IgM monoclonal antibody (Mab 36) which reacts with the circumsporozoite (CS) proteins of both P. falciparum and P. berghei was isolated from Plasmodium falciparum sporozoite-immunized mice. In assays of biological activity, Mab 36 induces the CS precipitation reaction with live sporozoites and blocks the invasion of hepatoma cells by sporozoites in vitro at concentrations much lower than those observed for previously reported CS protein-specific monoclonal antibodies. Mab 36 also provided complete protection against P. berghei sporozoite challenge in mice at low doses. Linear epitope mapping revealed that the epitope specificities recognized by Mab 36 are completely encompassed by other monoclonals previously shown to be associated in vivo with protection against P. falciparum or P. berghei sporozoite infection. These results suggest that the ability to make high-affinity IgM antibody to specific CS protein repeat epitopes may be important for eliciting protection against malarial infection.