Interferon-γ induces class II antigen expression on cultured rat mesangial smooth muscle cells

• Published in: Clinical immunology and immunopathology Vol. 53; no. 2; pp. 341 - 345
• Main Author: Mosquera, Jesús A.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.11.1989; New York, NY Academic Press; Boston
• Subjects: Animals; Antigens; Biological and medical sciences; Cells, Cultured; Fluorescent Antibody Technique; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Glomerular Mesangium - immunology; Histocompatibility antigens (hla, h-2 and other systems); Histocompatibility Antigens Class II - immunology; In Vitro Techniques; Interferon-gamma - pharmacology; Male; Molecular immunology; Muscle, Smooth - immunology; Rats; Rats, Inbred Lew; Recombinant Proteins; Cell culture; Rat; Mesangial cell; Major histocompatibility system; Rodentia; Class II histocompatibility antigen; Cytokine; Smooth muscle; Indirect immunofluorescence; In vitro; Renal glomerulus; Vertebrata; Mammalia; Urinary system; Fluorescence microscopy; Gamma interferon
• ISSN: 0090-1229; 1090-2341
• DOI: 10.1016/0090-1229(89)90062-7

In this study, Ia antigen expression was detected on cultured rat mesangial smooth muscle (MSM) cells by anti-Ia monoclonal antibodies and indirect immunofluorescence when the cultures were stimulated with recombinant interferon-γ. This finding may be relevant to the increased expression of Ia on mesangial cells during immunologically mediated glomerulonephritis, since it is possible that Ia expression may be induced not only on mesangial macrophages but also in MSM cells during immunologic pathogenesis of glomerular injury.