Failure of a helium-neon laser to affect components of wound healing in vitro

The red light of a helium-neon (He-Ne) laser has been reported to stimulate wound healing and cell growth. To investigate the nature of its influence on wound healing we have studied seven components of the healing process in vitro: human skin fibroblast, epithelial and endothelial cell proliferatio...

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Bibliographic Details
Published inBritish journal of dermatology (1951) Vol. 121; no. 2; p. 179
Main Authors Colver, G B, Priestley, G C
Format Journal Article
LanguageEnglish
Published England 01.08.1989
Subjects
Cell Division - radiation effects
Cell Movement - radiation effects
Cells, Cultured
Collagen - biosynthesis
Glycosaminoglycans - metabolism
Humans
Laser Therapy
Skin - cytology
Skin - metabolism
Skin - radiation effects
Wound Healing - radiation effects
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Summary:The red light of a helium-neon (He-Ne) laser has been reported to stimulate wound healing and cell growth. To investigate the nature of its influence on wound healing we have studied seven components of the healing process in vitro: human skin fibroblast, epithelial and endothelial cell proliferation, cellular migration from skin explants, collagen lattice contraction, collagen synthesis and glycosaminoglycans (GAG) secretion. We used a 5 mW He-Ne laser emitting a I mm diameter beam of wavelength 633 nm. Cellular proliferation was not affected by irradiation three times a day for 3 days. There was no effect on cellular migration or on the rate of collagen lattice contraction. The rate of collagen synthesis, measured as the incorporation of 3H-proline into collagenase-sensitive protein, was no greater than that of controls and GAG secretion did not increase in the irradiated group. We have not found any significant effects of He-Ne irradiation.
ISSN:0007-0963
DOI:10.1111/j.1365-2133.1989.tb01797.x