MR microscopy of cobalt-labeled nerve cells and pathways in an invertebrate brain (Sepia officinalis, Cephalopoda)

• Published in: Magnetic resonance in medicine Vol. 45; no. 4; pp. 575 - 579
• Main Authors: Quast, Michael J., Neumeister, Heike, Ezell, Edward L., Budelmann, Bernd U.
• Format: Journal Article
• Language: English
• Published: New York John Wiley & Sons, Inc 01.04.2001; Williams & Wilkins
• Subjects: Animals; Biological and medical sciences; Brain - cytology; brain anatomy; Cobalt; cuttlefish; Investigative techniques, diagnostic techniques (general aspects); magnetic resonance imaging (MRI); Magnetic Resonance Imaging - methods; Medical sciences; Microscopy - methods; Models, Animal; Mollusca; Nervous system; Neural Pathways - cytology; neuronal tracing; Neurons - cytology; Radiodiagnosis. Nmr imagery. Nmr spectrometry; Microscope; Evaluation; Anatomical variation; Cuttlefish; Central nervous system; Cobalt ion; Exploration; Experimental study; Cephalopoda; Iontophoresis; Neuron; Microscopy; Animal; Nuclear magnetic resonance; Technique; Mollusca; Invertebrata; Brain (vertebrata)
• ISSN: 0740-3194; 1522-2594
• DOI: 10.1002/mrm.1078

This article describes a novel application of contrast‐enhanced MR microscopy to trace nerve cells and pathways through small invertebrate brains. Using the cuttlefish Sepia officinalis (Cephalopoda) as a model, the cells and pathways of one of the brain nerves were labeled with paramagnetic cobalt(II) ions by conventional centripetal cobalt iontophoresis. In MR microscopy, the cobalt‐labeled cell bodies and pathways became hypointense in 9.4 T spin echo images. Their course and distribution were identical with those seen with conventional histological techniques after cobalt sulphide precipitation (with or without subsequent silver intensification). Magn Reson Med 45:575–579, 2001. © 2001 Wiley‐Liss, Inc.