A prospective controlled evaluation of TEST-yolk buffer in the preparation of sperm for human in vitro fertilization in suspected cases of male infertility

To evaluate sperm preincubation in tes and tris (TEST)-yolk buffer as a potential enhancing agent of fertilizing capacity of sperm during human in vitro fertilization (IVF). Oocytes obtained during IVF were divided into two groups: one group fertilized with TEST-yolk buffer-treated sperm and the oth...

Full description

Saved in:
Bibliographic Details
Published inFertility and sterility Vol. 58; no. 3; pp. 551 - 555
Main Authors Paulson, Richard J., Sauer, Mark V., Francis, Mary M., Macaso, Thelma M., Lobo, Rogerio A.
Format Journal Article Conference Proceeding
LanguageEnglish
Published New York, NY Elsevier Inc 01.09.1992
Elsevier Science
Subjects
Biological and medical sciences
Egg Yolk
Embryo Transfer
Female
Fertilization in Vitro
Gynecology. Andrology. Obstetrics
Humans
In vitro fertilization
Infertility, Male - therapy
Male
male factor
Medical sciences
Pregnancy
Prospective Studies
sperm
Spermatozoa - physiology
TEST-yolk buffer
Tromethamine
In vitro fertilization
TEST-yolk buffer
sperm
male factor
Binding
Spermatozoa
Prognosis
Semen
Exploration
Sterility
Genital diseases
Result
In vitro fertilization embryo transfer
Treatment
Immunological investigation
Preparation
Technique
Molecular biology
Male genital diseases
Online AccessGet full text

Cover

More Information
Summary:To evaluate sperm preincubation in tes and tris (TEST)-yolk buffer as a potential enhancing agent of fertilizing capacity of sperm during human in vitro fertilization (IVF). Oocytes obtained during IVF were divided into two groups: one group fertilized with TEST-yolk buffer-treated sperm and the other group with standard-prepared sperm. The University of Southern California IVF Program. Thirty-nine couples with suspected male factor infertility undergoing IVF. Preinsemination incubation of sperm in TEST-yolk buffer for 24hours. Fertilization rates in vitro. TEST-yolk buffer-treated sperm fertilized 140 of 241 oocytes (58%), whereas control sperm fertilized 108 of 251 oocytes (43%). Of four couples who had previously failed to fertilize during IVF, one fertilized with both specimens and three fertilized with TEST-yolk buffer-treated sperm only. TEST-yolk buffer pretreatment of sperm for 24hours results in higher fertilization rates during IVF among suspected male factor patients.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0015-0282
1556-5653
DOI:10.1016/S0015-0282(16)55262-5