Single-round isolation of diverse RNA aptamers from a random sequence pool

• Published in: Biology methods and protocols Vol. 3; no. 1; p. bpy004
• Main Authors: Imashimizu, Masahiko, Takahashi, Masaki, Amano, Ryo, Nakamura, Yoshikazu
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 2018
• Subjects: Methods Manuscript
• ISSN: 2396-8923; 2396-8923
• DOI: 10.1093/biomethods/bpy004

Abstract Aptamers are oligonucleotide ligands with specific binding affinity to target molecules. Generally, RNA aptamers are selected from an RNA pool with random sequences, using the technique termed SELEX, in which the target-binding RNA molecules are repeatedly isolated and exponentially amplified. Despite several advantages, SELEX often produces uncertain results during the iterative amplifications of the rare target-binding RNA molecules. Here, we develop a non-repeated, primer-less and target immobilization-free isolation method for generating RNA aptamers, which is robust to experimental noise. Uniquely, this method focuses on finding and removal of non-aptamer sequences from the RNA pool by RNase digestion leaving target-bound aptamer molecules, and thus is independent of aptamer types. The undigested RNA sequences remaining are so few in number that they must be mixed with a large excess of a known sequence for further manipulations and this sequence is then removed by restriction digestion followed by high-throughput sequencing analysis to identify aptamers. Using this method, we generated multiple RNA aptamers targeting α-thrombin and TGFβ1 proteins, independently. This method potentially generates thousands of sequences as aptamer candidates, which may enable us to predict a common average sequence or structural property of these aptamers that is different from input RNA.