Production of MLM-Type Structured Lipids Catalyzed by Immobilized Heterologous Rhizopus oryzae Lipase

• Published in: Journal of the American Oil Chemists' Society Vol. 88; no. 4; pp. 473 - 480
• Main Authors: Nunes, P. A, Pires-Cabral, P, Guillén, M, Valero, F, Luna, D, Ferreira-Dias, S
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.04.2011; Springer-Verlag; Springe-Verlag; Springer; Springer Nature B.V
• Subjects: Acidolysis; Agriculture; Biological and medical sciences; Biomaterials; Biotechnology; Capric acid; Caprylic acid; Chemistry; Chemistry and Materials Science; Enzymes; Fat industries; Fatty acids; Fish oils; Food industries; Food Science; Fundamental and applied biological sciences. Psychology; Industrial Chemistry/Chemical Engineering; Lipids; Olive oil; Original Paper; Rhizopus oryzae lipase; Solvents; Structured lipids; Yeasts; Capric acid; Caprylic acid; Structured lipids; Olive oil; Acidolysis; lipase; Structured lipid; Enzyme; Triacylglycerol lipase; Esterases; Vegetable oil; Edible oil; Carboxylic ester hydrolases; Oils and fats industry; Rhizopus oryzae lipase; Production; Hydrolases
• ISSN: 0003-021X; 1558-9331
• DOI: 10.1007/s11746-010-1702-y

This work aims to produce triacylglycerols (TAG) containing a medium-chain fatty acid (M) at positions sn-1,3 and a long-chain fatty acid (L) at sn-2 position, i.e. TAG of MLM type, by acidolysis of virgin olive oil with caprylic (C8:0) or capric (C10:0) acids, catalyzed by 1,3-selective Rhizopus oryzae heterologous lipase (rROL) immobilized in Eupergit® C and modified sepiolite. This lipase was produced by the methylotrophic yeast Pichia pastoris. Reactions were performed at 25 and 40 °C, for 24 h, either in solvent-free or in n-hexane media, at a molar ratio 1:2 (olive oil:free fatty acid). Higher incorporations of C8:0 (21.6 mol%) and C10:0 (34.8 mol%) into the TAG were attained in solvent-free media, at 40 °C, when rROL immobilized in Eupergit® C was used. In organic media, at 40 °C, only 15.9 and 14.1 mol%, incorporation of C8:0 or C10:0 were, respectively observed. Lower incorporations were attained for both acids (3.4-7.0 mol%) when native ROL (nROL) in both supports and rROL in modified sepiolite were used. rROL in Eupergit® C maintained its activity during the first four or three 23-h batches, respectively when C8:0 (half-life time, t ₁/₂ = 159 h) or C10:0 (t ₁/₂ = 136 h) were used, decreasing thereafter following a time delay model.