Assessment of postprandial hepatic glycogen synthesis from uridine diphosphoglucose kinetics in obese and lean non-diabetic subjects

• Published in: International Journal of Obesity Vol. 24; no. 10; pp. 1297 - 1302
• Main Authors: Paquot, N, Schneiter, P, Scheen, A.J, Lefebvre, P.J, Tappy, L
• Format: Journal Article Web Resource
• Language: English
• Published: England Nature Publishing Group 01.10.2000
• Subjects: Adult; Aged; Analgesics; analysis; biosynthesis; blood; Blood Glucose; Blood Glucose - analysis; Carbohydrates; complications; Diabetes; Endocrinologie, métabolisme & nutrition; Endocrinology, metabolism & nutrition; Fatty Acids, Nonesterified; Fatty Acids, Nonesterified - blood; Female; Glucose; Glucose Intolerance; Glucose Intolerance - complications; Glucose Intolerance - metabolism; Glucose Intolerance/complications/metabolism; glucose tolerance; glycogen; Homeostasis; Human health sciences; Humans; Hyperglycemia; Ingestion; Insulin; Insulin - blood; Insulin Resistance; Isotopic enrichment; Kinetics; Liver; Liver - metabolism; Liver Glycogen; Liver Glycogen - biosynthesis; Male; metabolic diseases; Metabolism; Middle Aged; monitoring; Musculoskeletal system; Obesity; Obesity - complications; Obesity - metabolism; Obesity/complications/metabolism; patients; pharmacokinetics; Physiology; Postprandial Period; Sciences de la santé humaine; skeletal muscle; Time Factors; uridine; Uridine Diphosphate Glucose; Uridine Diphosphate Glucose - metabolism; Uridine Diphosphate Glucose - pharmacokinetics; Uridine Diphosphate Glucose/metabolism/pharmacokinetics; Switzerland
• ISSN: 0307-0565; 1476-5497; 1476-5497
• DOI: 10.1038/sj.ijo.0801386

BACKGROUND: Obese patients are frequently characterized by insulin resistance and decreased insulin-mediated glycogen synthesis in skeletal muscle. Whether they also have impaired postprandial hepatic glycogen synthesis remains unknown. AIM: To determine whether postprandial hepatic glycogen synthesis is decreased in obese patients compared to lean subjects. METHODS: Lean and obese subjects with impaired glucose tolerance were studied over 4 h after ingestion of a glucose load. Hepatic uridine diphosphoglucose kinetics were assessed using 13C-galactose infusion, with monitoring of urinary acetaminophen-glucuronide isotopic enrichment to estimate hepatic glycogen kinetics. RESULTS: Estimated net hepatic glycogen synthesis amounted to 18.6 and 22.6% of the ingested load in lean and obese subjects, respectively. CONCLUSION: Postprandial hepatic glycogen metabolism is not impaired in non-diabetic obese subjects.