Heat-Shock Mannoproteins as Targets of Secretory IgA in Candida albicans

• Published in: The Journal of infectious diseases Vol. 169; no. 6; pp. 1401 - 1405
• Main Authors: Polonelli, L., Gerloni, M., Conti, S., Fisicaro, P., Cantelli, C., Portincasa, P., Almondo, F., Barea, P. L., Hernando, F. L., Ponton, J.
• Format: Journal Article
• Language: English
• Published: Chicago, IL The University of Chicago Press 01.06.1994; University of Chicago Press
• Subjects: Antibodies, Fungal - immunology; Biological and medical sciences; Blotting, Western; Candida albicans; Candidiasis; Candidiasis - immunology; Candidiasis, Oral - immunology; Candidiasis, Vulvovaginal - immunology; Concise Communications; Ent and stomatologic mycoses; Epithelial cells; Epitopes; Female; Fluorescent Antibody Technique; Heat-Shock Proteins - immunology; Human mycoses; Humans; Immunoglobulin A, Secretory - immunology; Infectious diseases; Medical sciences; Membrane Glycoproteins - immunology; Monoclonal antibodies; Mycoses; Oxidation; Reactivity; Saliva; Temperature; Washing; Yeasts; Candida albicans; Human; Candidiasis; IgA; Mycosis; Antigenic determinant; Stomatology; Gene expression; In vitro; Female genital diseases; Fungi; Infection; Immunological investigation; Heat shock protein; Immunoblotting assay; Fungi Imperfecti; Thallophyta
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1093/infdis/169.6.1401

The expression of Candida albicans antigenic determinants reacting with secretory IgA from patients with oral and vaginal candidiasis was investigated under different in vitro conditions. Reversible antigenic transitions were inducible in synthetic medium by temperature shifts, as the yeast cells were positive by an indirect immunofluorescence assay after being incubated at 37°C but not at 25°C. In vitro temperature-inducible C. albieans antigenic determinants reactive with secretory IgA were characterized by radioimmune Western blot as mannoproteins with molecular masses of 180–200, 130–150, 90–110, and 60–70 kDa. This is the first report on the expression of mannoproteins regulated by temperature involved in the secretory immune response during mucosal candidiasis.