Differential cytolocalization of prosomes in axolotl during oogenesis and meiotic maturation

The prosomes, a novel type of small RNA-protein complex previously characterized in avian and mammalian cells, were isolated from axolotl (Ambystoma mexicanum) oocytes and identified by sedimentation analysis and protein composition. The prosomal nature of these particles was further ascertained by...

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Published inJournal of cell science Vol. 90; no. 4; pp. 543 - 553
Main Authors GAUTIER, J, PAL, J. K, GROSSI DE SA, M.-F, BEETSCHEN, J. C, SCHERRER, K
Format Journal Article
LanguageEnglish
Published Cambridge Company of Biologists 01.08.1988
Subjects
Ambystoma mexicanum
Animals
Antigens - analysis
Biological and medical sciences
Cell cycle, cell proliferation
Cell physiology
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Immunoblotting
Meiosis
Microscopy, Fluorescence
Microscopy, Phase-Contrast
Molecular and cellular biology
Oocytes - analysis
Oocytes - cytology
Oogenesis
Proteins - analysis
RNA, Messenger - analysis
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Summary:The prosomes, a novel type of small RNA-protein complex previously characterized in avian and mammalian cells, were isolated from axolotl (Ambystoma mexicanum) oocytes and identified by sedimentation analysis and protein composition. The prosomal nature of these particles was further ascertained by immunoblot analysis with anti-duck prosome monoclonal antibodies. By in vitro [35S]methionine labelling, de novo synthesis of prosomal proteins could be detected neither during oogenesis nor meiotic maturation. The results obtained by both indirect immunofluorescence and immunoblot analyses demonstrated a dramatic change in the localization of prosomal antigens during oocyte development. They were initially detected in the oocyte cytoplasm, during oocyte growth. At the end of vitellogenesis (stages V-VI), they entered the nucleus (germinal vesicle) and were accumulated there to the highest concentration. During oocyte maturation, after nuclear envelope breakdown, prosomal antigens were found to be localized again in the cytoplasm, until fertilization. No specific localization of prosomal antigens in mature oocytes, unfertilized and fertilized eggs was observed within the oocyte cytoplasm in relation to the cytoplasmic rearrangements leading to grey crescent formation.
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ISSN:0021-9533
1477-9137
DOI:10.1242/jcs.90.4.543