Rapid modification of retroviruses using lipid conjugates

Methods are needed to manipulate natural nanoparticles. Viruses are particularly interesting because they can act as therapeutic cellular delivery agents. Here we examine a new method for rapidly modifying retroviruses that uses lipid conjugates composed of a lipid anchor (1,2-distearoyl-sn-glycero-...

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Bibliographic Details
Published inNanotechnology Vol. 20; no. 6; pp. 065103 - 065103 (10)
Main Authors Mukherjee, Nimisha G, Andrew Lyon, L, Doux, Joseph M Le
Format Journal Article
LanguageEnglish
Published England IOP Publishing 11.02.2009
Subjects
Cell Line
Humans
Phospholipids - metabolism
Retroviridae - chemistry
Streptavidin - metabolism
Virus Attachment
Virus Physiological Phenomena
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Summary:Methods are needed to manipulate natural nanoparticles. Viruses are particularly interesting because they can act as therapeutic cellular delivery agents. Here we examine a new method for rapidly modifying retroviruses that uses lipid conjugates composed of a lipid anchor (1,2-distearoyl-sn-glycero-3-phosphoethanolamine), a polyethylene glycol chain, and biotin. The conjugates rapidly and stably modified retroviruses and enabled them to bind streptavidin. The implication of this work for modifying viruses for gene therapy and vaccination protocols is discussed.
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ISSN:0957-4484
1361-6528
DOI:10.1088/0957-4484/20/6/065103