Correlation of Allergen-Induced IL-5 and IL-13 Production by Peripheral Blood T Cells of Asthma Patients

• Published in: International Archives of Allergy and Immunology Vol. 134; no. Suppl 1; pp. 7 - 11
• Main Authors: Hashimoto, Tomomi, Akiyama, Kazuo, Kawaguchi, Hiroshi, Maeda, Yuji, Taniguchi, Masami, Kobayashi, Noriaki, Mori, Akio
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Basel, Switzerland Karger 01.06.2004; S. Karger AG
• Subjects: Adult; Allergens - immunology; Allergic diseases; Allergies; Antigens, Dermatophagoides - immunology; Asthma; Asthma - immunology; Biological and medical sciences; Cells, Cultured; Cytokines; Dermatitis, Atopic - immunology; Enzyme-Linked Immunosorbent Assay; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; Immunopathology; Interleukin-13 - biosynthesis; Interleukin-4 - biosynthesis; Interleukin-5 - biosynthesis; Medical sciences; Original Paper; Skin allergic diseases. Stinging insect allergies; T cell receptors; T-Lymphocytes - metabolism; IL-5; Bronchial asthma; IL-13; Atopic dermatitis; T cell; Human; Lung disease; Allergy; Immunopathology; Skin disease; Respiratory disease; Cytokine; Interleukin 13; Respiratory system; Atopy; Asthma; Respiratory tract; Blood cell; Immunology; Interleukin 5; T-Lymphocyte; Bronchus disease; Obstructive pulmonary disease; Allergen
• ISBN: 9783805577687; 3805577680
• ISSN: 1018-2438; 1423-0097
• DOI: 10.1159/000077786

Background: Helper T cells and T cell cytokines are implicated in allergic disorders such as asthma and atopic dermatitis (AD). We reported enhanced interleukin-5 (IL-5) production by peripheral blood T cells of asthmatic patients. Production of cytokines, particularly IL-5 and IL-13, by peripheral blood mononuclear cells (PBMC) obtained from bronchial asthma (BA) and AD patients was investigated in the present study. Methods: PBMC were cultured in the presence of either polyclonal activator, phorbol ester plus Ca 2+ ionophore or Dermatophagoidesfarinae (Df) antigen. The resulting supernatants were assayed for IL-4, IL-5, and IL-13 by specific ELISAs. Results: IL-5 and IL-13 production in response to Df antigen is significantly higher in allergic groups compared to control subjects. IL-5 responses induced by Df antigen were strongly correlated with IL-13 responses. Conclusion: In spite of IL-13 gene proximity to IL-4 gene, IL-5 production was more strongly correlated to IL-13 production in the BA group, suggesting a common control mechanism that regulates the IL-5 and IL-13 gene.