Genetic Analysis of Herpes Simplex Virus Type 1 Isolates from Recurrent Lesions and Clinical Reinfections

• Published in: The Journal of infectious diseases Vol. 172; no. 6; pp. 1602 - 1605
• Main Authors: Rojas, José M., Sánchez-Palomino, Sonsoles, Santana, Milagros, López-Galíndez, Cecilio, Tabarés, Enrique
• Format: Journal Article
• Language: English
• Published: Chicago, IL The University of Chicago Press 01.12.1995; University of Chicago Press
• Subjects: Biological and medical sciences; Concise Communications; DNA; Female; Genetic variation; Herpes Simplex - virology; herpes simplex virus 1; Herpesvirus 1, Human - genetics; Human herpesvirus 1; Human viral diseases; Humans; Infectious diseases; Lesions; Lips; Medical genetics; Medical sciences; Recurrence; Reinfection; Restriction endonuclease analysis; Ribonuclease, Pancreatic - pharmacology; Simplexvirus; Thymidine Kinase - genetics; Viral diseases; Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye; Viral Envelope Proteins - genetics; Viruses; Human; Relapse; Genetic variability; Enzyme; Pathogenesis; Herpesviridae; Alphaherpesvirinae; Herpes; Epidemiology; Infection; Virus; Herpesvirus hominis 1; Viral disease; Restriction endonuclease
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1093/infdis/172.6.1602

A study of the genetic variability of herpes simplex virus (HSV) type 1 from recurrent lesions and clinical reinfections was done using restriction endonuclease analysis and the RNase A mismatch cleavage method. Comparative genetic analyses of HSV-1 recurrent isolates from 1 patient and of HSV-1 isolates from different anatomic areas (vagina and lip) from another patient showed differences only in the glycoprotein B gene but not in the thymidine kinase gene even though the viruses had the same restriction endonuclease pattern. These results suggest the RNase A mismatch cleavage method is useful for epidemiologic studies of DNA viruses.