Effect of heme oxygenase-1 on renal function in rats with liver cirrhosis

• Published in: World journal of gastroenterology : WJG Vol. 17; no. 3; pp. 322 - 328
• Main Authors: Guo, Shi-Bin, Duan, Zhi-Jun, Li, Qing, Sun, Xiao-Yu
• Format: Journal Article
• Language: English
• Published: United States Baishideng Publishing Group Co., Limited 21.01.2011
• Subjects: Animals; Enzyme Inhibitors - pharmacology; Heme Oxygenase-1 - antagonists & inhibitors; Heme Oxygenase-1 - genetics; Heme Oxygenase-1 - metabolism; Hemodynamics; Hepatorenal Syndrome - pathology; Hepatorenal Syndrome - physiopathology; Humans; Kidney - drug effects; Kidney - metabolism; Kidney Function Tests; Liver Cirrhosis - pathology; Liver Cirrhosis - physiopathology; Male; mRNA表达; Original; Protoporphyrins - pharmacology; Random Allocation; Rats; Rats, Sprague-Dawley; RNA, Messenger - metabolism; 肝硬化; 肾功能; 蛋白表达; 血红素氧化酶-1; 逆转录聚合酶链反应; 锌原卟啉; Cobalt protoporphyrin; Bile duct ligation; Heme oxygenase-1; Biliary cirrhosis; Zinc protoporphyrin IX; Carbon monoxide; Hepatorenal syndrome
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v17.i3.322

AIM： To investigate the role of heme oxygenase-1 （HO-1） in pathogenesis of experimental hepatorenal syndrome （HRS）. METHODS： Rats were divided into liver cirrhotic group, zinc protoporphyrin IX （ZnPP） treatment group, cobalt protoporphyrin （CoPP） treatment group and sham group. Biliary cirrhosis was established by bile duct ligation in the first three groups. Rats in the ZnPP and CoPP treatment groups received intraperitoneal injection of ZnPP and CoPP, respectively, 24 h before sample collection. Expression of HO-1 mRNA in kidney was detected by reverse-transcription polymerase chain reaction, while protein expression was determined by immunohis-tochemical analysis. Hematoxylin and eosin staining was performed to observe liver cirrhosis and renal structure. Renal artery blood flow, mean arterial pressure and portal vein pressure, 24 h total urinary volume, serum and urine sodium concentrations, and creatinine clearance rate （Ccr） were also measured.RESULTS： The HO-1 mRNA and protein expression levels in kidney, 24 h total urinary volume, renal artery blood flow, serum and urine sodium concentration and Ccr were lower in cirrhotic group than in sham group （P 0.05）. However, they were significantly lower in ZnPP treatment group than in cirrhotic group and significantly higher in CoPP treatment group than in cirrhotic group （P 0.05）. CONCLUSION： Low HO-1 expression level in kidney is an important factor for experimental HRS.