Stimulation of fecal bacteria in ambient waters by experimental inputs of organic and inorganic phosphorus

• Published in: Water research (Oxford) Vol. 47; no. 10; pp. 3455 - 3466
• Main Authors: Chudoba, Elizabeth A., Mallin, Michael A., Cahoon, Lawrence B., Skrabal, Stephen A.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 15.06.2013; Elsevier
• Subjects: Animals; Applied sciences; Bacteria - drug effects; Bacteria - growth & development; Bacterial growth rate; Bacteriological Techniques - methods; bioassays; coliform bacteria; constructed wetlands; Continental surface waters; Dilutions; Enterobacteriaceae - drug effects; Enterobacteriaceae - growth & development; Environmental Monitoring - methods; Exact sciences and technology; farms; Fecal bacteria; Feces - microbiology; gardens; grazing; human health; Humans; inorganic phosphorus; microbial growth; Microzooplankton grazing; Natural water pollution; nitrogen; Nitrogen - metabolism; Nitrogen - pharmacology; North Carolina; Nutrients; phosphorus; Phosphorus - metabolism; Phosphorus - pharmacology; Pollution; pollution load; stormwater; urban areas; urbanization; Water Microbiology; Water Pollution - prevention & control; Water treatment and pollution; Wetlands; Zooplankton - physiology; North Carolina; Nutrients; Fecal bacteria; Dilutions; Microzooplankton grazing; Bacterial growth rate; Growth; Stimulation; Coliforms; Phosphorus Compounds; Phosphorus Organic compounds; Plankton; Artificial medium; Surface water; Bacteria; Water pollution; Feces; Biological contamination; Wetland; Zooplankton
• ISSN: 0043-1354; 1879-2448
• DOI: 10.1016/j.watres.2013.03.047

Fecal microbial pollution of recreational and shellfishing waters is a major human health and economic issue. Microbial pollution sourced from stormwater runoff is especially widespread, and strongly associated with urbanization. However, non-point source nutrient pollution is also problematic, and may come from sources different from fecal-derived pollution (i.e. fertilization of farm fields, lawns and gardens, and ornamental urban areas). Fecal bacteria require nutrients; thus the impact of such nutrient loading on survival and abundance of fecal coliform bacteria in ambient waters was experimentally investigated in a constructed wetland in coastal North Carolina, USA. A series of nutrient-addition bioassays testing impacts of inorganic and organic nitrogen and phosphorus demonstrated that additions of neither organic nor inorganic nitrogen stimulated fecal coliform bacteria. However, phosphorus additions provided significant stimulation of fecal coliform growth at times; on other occasions such additions did not. Dilution bioassays combined with nutrient additions were subsequently devised to assess potential impacts of microzooplankton grazing on the target fecal bacteria populations. Results demonstrated grazing to be a significant bacterial reduction factor in 63% of tests, potentially obscuring nutrient effects. Thus, combining dilution experiments with nutrient addition bioassays yielded simultaneous information on microzooplankton grazing rates on fecal bacteria, fecal bacterial growth rates, and nutrient limitation. Overall, when tested against a non-amended control, additions of either organic or inorganic phosphorus significantly stimulated fecal coliform bacterial growth on 50% of occasions tested, with organic phosphorus generally providing greater stimulation. The finding of significant phosphorus stimulation of fecal bacteria indicates that extraneous nutrient loading can, at times, augment the impacts of fecal microbial pollution of shellfishing and human contact waters. [Display omitted] •Microzooplankton grazing reduces fecal bacteria counts and skews bioassay results.•Dilutions plus nutrient additions measure both grazing and nutrient input impacts.•Phosphorus additions significantly stimulated fecal bacteria growth in ambient water.•Nitrogen additions failed to significantly stimulate fecal bacterial growth.