Intradermal AQP4 peptide immunization induces clinical features of neuromyelitis optica spectrum disorder in mice
We challenged to create a mouse model of neuromyelitis optica spectrum disorder (NMOSD) induced by AQP4 peptide immunization. Intradermal immunization with AQP4 p201–220 peptide induced paralysis in C57BL/6J mice, but not in AQP4 KO mice. AQP4 peptide-immunized mice showed pathological features simi...
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Published in | Journal of neuroimmunology Vol. 380; p. 578109 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
15.07.2023
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Subjects | |
Online Access | Get full text |
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Summary: | We challenged to create a mouse model of neuromyelitis optica spectrum disorder (NMOSD) induced by AQP4 peptide immunization. Intradermal immunization with AQP4 p201–220 peptide induced paralysis in C57BL/6J mice, but not in AQP4 KO mice. AQP4 peptide-immunized mice showed pathological features similar to NMOSD. Administration of anti-IL-6 receptor antibody (MR16–1) inhibited the induction of clinical signs and prevented the loss of GFAP/AQP4 and deposition of complement factors in AQP4 peptide-immunized mice. This novel experimental model may contribute to further understanding the pathogenesis of NMOSD, elucidating the mechanism of action of therapeutic agents, and developing new therapeutic approaches.
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•Intradermal immunization with AQP4 peptide at multiple sites induced paralysis in mice.•Intradermal immunization with AQP4 peptide decreased GFAP/AQP4 expression in mice.•Intradermal immunization with AQP4 peptide induced anti-AQP4 autoantibody in mice.•Anti-IL-6 receptor antibody prevented the development of paralysis in this mouse model. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0165-5728 1872-8421 |
DOI: | 10.1016/j.jneuroim.2023.578109 |