Characterization of Dengue Virus Serotype 2 Cosmopolitan Genotype Circulating in Colombia

Dengue virus (DENV) is the etiological agent of dengue fever (DF), which is among the most prevalent vector-borne diseases in the tropics. In 2022, the Colombian health surveillance system reported more than 69,000 cases of DF. As part of a hospital-based fever surveillance study, acute-phase sera w...

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Published inThe American journal of tropical medicine and hygiene Vol. 109; no. 6; pp. 1298 - 1302
Main Authors Ciuoderis, Karl A., Usuga, Jaime, Moreno, Isabel, Perez-Restrepo, Laura S., Flórez, Diana Y., Cardona, Andres, Cloherty, Gavin A., Berg, Michael G., Hernandez-Ortiz, Juan P., Osorio, Jorge E.
Format Journal Article
LanguageEnglish
Published United States Institute of Tropical Medicine 06.12.2023
The American Society of Tropical Medicine and Hygiene
Subjects
Colombia - epidemiology
Dengue
Dengue fever
Dengue Virus
Epidemiology
Genomes
Genotype
Genotype & phenotype
Health surveillance
Humans
Phylogenetics
Phylogeny
Serogroup
Short Report
Whole genome sequencing
Colombia
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Summary:Dengue virus (DENV) is the etiological agent of dengue fever (DF), which is among the most prevalent vector-borne diseases in the tropics. In 2022, the Colombian health surveillance system reported more than 69,000 cases of DF. As part of a hospital-based fever surveillance study, acute-phase sera were collected from 4,545 patients with suspected dengue between 2020 and 2023 in three municipalities of Colombia. Combined reverse transcription–polymerase chain reaction and antigen rapid testing confirmed that 376 patients (8.3%) had DF. The virus was isolated in cell culture from 166 of these patients (44.1%), and genome sequencing was performed successfully on 122 (73.5%). Three DENV serotypes (1, 2, and 3) were identified. Phylogenetic analyses of the DENV-2 sequences revealed that 42 of 50 of the isolates (84%) belonged to the DENV-2 cosmopolitan genotype lineage, clustering with sequences from Asia, Peru, and Brazil. We report the detection, isolation, and whole-genome sequencing (11 Kb) of the DENV-2 cosmopolitan genotype and its recent introduction to Colombia.
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Disclosure: G. A. C. and M. G. B. are Abbott employees and shareholders. J. O. is the single owner of Vaccigen, LLC. J. E. O. and J. P. H. O. are employees and shareholders of VaxThera. This study was approved by the Research and Ethics Committee of the Corporación Investigaciones Biológicas (No. 0822JHO04). Voluntary participation in this study occurred after signing written informed consent. For participants younger than 18 years, minor assent was also obtained, and parents or a legal representative provided written informed consent on their behalf.
Authors’ addresses: Karl A. Ciuoderis, Jaime Usuga, Isabel Moreno, Laura S. Perez-Restrepo, Diana Y. Flórez, and Andres Cardona, GHI One Health Colombia, Universidad Nacional de Colombia, Medellín, Colombia, E-mails: coord_cwohc@unal.edu.co, jausugar@unal.edu.co, imorenol@unal.edu.co, diflorezc@unal.edu.co, lab_cwohc@unal.edu.co, and ancardona@unal.edu.co. Gavin A. Cloherty and Michael G. Berg, Infectious Diseases Research, Abbott Diagnostics, Abbott Park, IL, E-mails: gavin.cloherty@abbott.com and michael.berg@abbott.com. Juan P. Hernández-Ortiz, GHI One Health Colombia, Universidad Nacional de Colombia, Medellín, Colombia, and Department of Materials and Nanotechnology, Universidad Nacional de Colombia, Medellin, Colombia, E-mail: jphernandezo@unal.edu.co. Jorge E. Osorio, Department of Pathobiological Sciences, School of Veterinary Medicine, and Global Health Institute, University of Wisconsin, Madison, WI, E-mail: jorge.osorio@wisc.edu.
ISSN:0002-9637
1476-1645
1476-1645
DOI:10.4269/ajtmh.23-0375