Enhancement by Microencapsulation of Rotavirus-Specific Intestinal Immune Responses in Mice Assessed by Enzyme-Linked Immunospot Assay and Intestinal Fragment Culture

• Published in: The Journal of infectious diseases Vol. 171; no. 5; pp. 1334 - 1338
• Main Authors: Brown, Kurt A., Moser, Charlotte A., Speaker, Tully J., Khoury, Christian A., Kim, Joon E., Offit, Paul A.
• Format: Journal Article
• Language: English
• Published: Chicago, IL The University of Chicago Press 01.05.1995; University of Chicago Press
• Subjects: Alginates; Animals; Animals, Suckling; Antibodies; Antibodies, Viral - biosynthesis; Biological and medical sciences; Capsules; Chondroitin Sulfates; Concise Communications; Culture Techniques; Drug Carriers; Drug Compounding; Enzyme-Linked Immunosorbent Assay - methods; Glucuronic Acid; Hexuronic Acids; Human viral diseases; Humoral immunity; Immune response; Immunoglobulin A - biosynthesis; Infectious diseases; Inoculation; Intestines - immunology; Lymphoid Tissue - immunology; Medical sciences; Mice; Microencapsulation; Plasma cells; Rotavirus; Rotavirus - immunology; Spermine; Vaccination; Viral diseases; Viral diseases of the digestive system; Viruses; Encapsulation; Immunization; Immune response; Rotavirus; Antibody; Rodentia; Gut; Lymphoid tissue; Experimental study; Reoviridae; Virus; Vertebrata; Mammalia; Mouse; Immunological investigation; Animal; Humoral immunity
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1093/infdis/171.5.1334

The capacity of microencapsulation to enhance the humoral immune response to rotavirus in the gut-associated lymphoid tissue (GALT) of mice was determined by using a system of microencapsulation based on the ionic linkage of aqueous anionic polymers and an aqueous amine. Inoculation of mice with microencapsulated rotavirus enhanced the frequencies of virus-specific IgA-secreting cells in the lamina propria as well as the quantities of virus-specific IgA produced in GALT. In addition, an enhanced virus-specific immune response was associated with enhanced production of presumably polyclonal, non-rotavirus-specific antibodies in GALT. The mechanism by which microencapsulation enhances the humoral immune response remains to be determined.