A simple mutagenesis using natural competence in Tannerella forsythia

We report the discovery of natural competence in Tannerella forsythia and its application to targeted chromosomal mutagenesis. Keeping T. forsythia in a biofilm throughout the procedure allowed efficient DNA uptake and allelic replacement. This simple method is cost-effective and reproducible compar...

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Bibliographic Details
Published inJournal of microbiological methods Vol. 94; no. 3; pp. 378 - 380
Main Authors Nishikawa, Kiyoshi, Tanaka, Yoshinobu
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.09.2013
Subjects
Bacteroides forsythus
Bacteroidetes - genetics
Bacteroidetes - metabolism
Bacteroidetes - physiology
biofilm
Biofilms
Cell Culture Techniques
cost effectiveness
culture media
DNA
DNA - metabolism
electroporation
Genes, Bacterial
Molecular Biology - methods
Mutagenesis
Natural competence
Plasmids
Tannerella forsythia
Transformation
Transformation, Bacterial
Tannerella forsythia
Transformation
Natural competence
Mutagenesis
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Summary:We report the discovery of natural competence in Tannerella forsythia and its application to targeted chromosomal mutagenesis. Keeping T. forsythia in a biofilm throughout the procedure allowed efficient DNA uptake and allelic replacement. This simple method is cost-effective and reproducible compared with the conventional protocols using broth culture and electroporation. •We discover natural competence in a periodontal pathogen, Tannerella forsythia.•We develop a simple method for targeted mutagenesis in Tannerella forsythia•Keeping T. forsythia in biofilm throughout the procedures enhances transformation.•Freshness of biofilm is critical for efficient transformation.
Bibliography:http://dx.doi.org/10.1016/j.mimet.2013.07.011
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ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2013.07.011