A polymorphic, telomeric-like sequence microsatellite in the Neotropical fish Prochilodus

A microsatellite locus from the Neotropical fish genus Prochilodus was isolated using PCR-based isolation of microsatellite arrays. Of 470 positive clones, 15 were sequenced, and 10 of them showed an (AATTT) n repeat. Primers were designed, and analysis of polymorphism revealed 11 alleles in three P...

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Published inCytogenetic and genome research Vol. 98; no. 4; pp. 308 - 310
Main Authors Hatanaka, T., Henrique-Silva, F., Galetti Jr, P.M.
Format Journal Article
LanguageEnglish
Published Basel, Switzerland Karger 01.01.2002
S. Karger AG
Subjects
Animal Cytogenetics and Comparative Mapping
Animals
Biological and medical sciences
Cytogenetics
Fishes - genetics
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
In Situ Hybridization, Fluorescence
Methods and miscellaneous
Microsatellite Repeats - genetics
Prochilodus
Species Specificity
Telomere - genetics
Tropical Climate
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Summary:A microsatellite locus from the Neotropical fish genus Prochilodus was isolated using PCR-based isolation of microsatellite arrays. Of 470 positive clones, 15 were sequenced, and 10 of them showed an (AATTT) n repeat. Primers were designed, and analysis of polymorphism revealed 11 alleles in three Prochilodus species. Fluorescence in situ hybridization analysis showed signals predominantly in the telomeric regions of several chromosomes. The description of this microsatellite may contribute to studies of the population structure of this fish group.   
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ISSN:1424-8581
1424-859X
DOI:10.1159/000071054