Isolation and molecular characterization of potentially pathogenic Acanthamoeba genotypes from diverse water resources including household drinking water from Khyber Pakhtunkhwa, Pakistan

• Published in: Parasitology research (1987) Vol. 112; no. 8; pp. 2925 - 2932
• Main Authors: Tanveer, Tania, Hameed, Abdul, Muazzam, Ambreen Gul, Jung, Suk-Yul, Gul, Asma, Matin, Abdul
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2013; Springer
• Subjects: Acanthamoeba; Acanthamoeba - genetics; Acanthamoeba - pathogenicity; Aquatic microbiology; Biomedical and Life Sciences; Biomedicine; Contamination; Drinking water; Drinking Water - parasitology; Family Characteristics; Genetic aspects; Genotype; Health aspects; Humans; Immunology; Medical Microbiology; Microbiology; Molecular genetics; Original Paper; Pakistan; Physiological aspects; Protozoa; Water; Water - parasitology; Pakistan; Single Polymerase Chain Reaction Product; Acanthamoeba Isolation; Acanthamoeba Species; Keratitis; Acanthamoeba Keratitis
• ISSN: 0932-0113; 1432-1955
• DOI: 10.1007/s00436-013-3465-5

Acanthamoeba , an opportunistic protozoan pathogen, is ubiquitous in nature, and therefore plays a predatory role and helps control microbial communities in the ecosystem. These Acanthamoeba species are recognized as opportunistic human pathogens that may cause blinding keratitis and rare but fatal granulomatous encephalitis. To date, there is not a single report demonstrating Acanthamoeba isolation and identification from environmental sources in Pakistan, and that is the aim of this study. Acanthamoeba were identified by morphological characteristics of their cysts on non-nutrient agar plates seeded with Escherichia coli . Additionally, the polymerase chain reaction (PCR) was performed with genus-specific primers followed by direct sequencing of the PCR product for molecular identification. Furthermore, our PCR and sequencing results confirmed seven different pathogenic and nonpathogenic genotypes, including T2–T10, T4, T5, T7, T15, T16, and T17. To the best of our knowledge, we have identified and isolated Acanthamoeba sp., for the first time, from water resources of Khyber Pakhtunkhwa, Pakistan. There is an urgent need to address (1) the pathogenic potential of the identified genotypes and (2) explore other environmental sources from the country to examine the water quality and the current status of Acanthamoeba species in Pakistan, which may be a potential threat for public health across the country.